Document Detail

Molecular detection of black-pigmented bacteria in infections of endodontic origin.
MedLine Citation:
PMID:  11556558     Owner:  NLM     Status:  MEDLINE    
A 16S rDNA-directed polymerase chain reaction method was used to assess the occurrence of four black-pigmented anaerobic rods in root canal infections. Samples were obtained from 54 infected teeth. Ten cases were diagnosed as acute periradicular abscesses. DNA was extracted from the samples and analyzed using a polymerase chain reaction-based identification assay. The method allowed detection of black-pigmented bacteria anaerobes in 59.3% of the examined teeth. Twelve cases yielded more than one black-pigmented species. In general Porphyromonas endodontalis was found in 42.6%, Porphyromonas gingivalis in 27.8%, Prevotella nigrescens in 7.4%, and Prevotella intermedia in 5.6% of the cases. P. endodontalis was found in 70% of the pus samples, P. gingivalis in 40%, and P. intermedia in 10%. P. gingivalis was always found associated with P. endodontalis in abscessed teeth. P. nigrescens was not found in any pus sample. The high prevalence of P. endodontalis and P. gingivalis suggests that they can play an important role in the pathogenesis of periradicular diseases.
J F Siqueira; I N Rôças; J C Oliveira; K R Santos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of endodontics     Volume:  27     ISSN:  0099-2399     ISO Abbreviation:  J Endod     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-14     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  563-6     Citation Subset:  D    
Department of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
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MeSH Terms
Bacterial Typing Techniques
Bacteroidaceae Infections / microbiology*
DNA, Bacterial / analysis
Periapical Abscess / microbiology*
Polymerase Chain Reaction
Porphyromonas / genetics,  isolation & purification*,  pathogenicity
Prevotella / genetics,  isolation & purification*,  pathogenicity
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Ribosomal, 16S

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