Document Detail

Molecular detection of adeno-associated virus in cases of spontaneous and intentional human abortion.
MedLine Citation:
PMID:  20827766     Owner:  NLM     Status:  MEDLINE    
Pregnancy failure is a common event and often of unknown cause. Some viruses are thought to cause abortions including the adeno-associated viruses (AAV), viruses which are regarded as being without any definitive association to any human disease. This study investigated AAV infection in 81 human abortions, both spontaneous and intentional that occurred up to the 23rd week of gestation. Nucleic acid of AAV-2, 3, and 5 types from 118 decidual and chorionic tissues, collected from the patients in this study, was amplified by nested-PCR. In situ hybridization (ISH) was developed with a digoxigenin-labeled AAV probe in paraffin embedded tissues from the AAV positive cases. AAV was observed in 28.4% (23/81) of the cases, of which, 78.3% (18/23) were in the decidua and 21.7% (5/23) in the extravillous trophoblast, the chorionic plate, or chorionic villi fragments. AAV-2, the only type detected, occurred in 32.3% (22/68) and in 7.7% (1/13) of the spontaneous and intentional abortions, respectively. ISH revealed AAV in the decidua, chorionic tissue or chorionic plate and extravillous trophoblast. The detection of only AAV-2 type indicates that it is the most frequent in the population studied and/or shows tissue tropism. The presence of AAV in decidual or trophoblastic cells in cases of abortion, as observed by ISH, implies that the virus could jeopardize the pregnancy. The significant predominance in spontaneous cases suggests possibly a causal association between AAV and abortion.
Christiane Curi Pereira; Luciana Bueno de Freitas; Paulo Roberto Merçon de Vargas; Maria Luiza Borges de Azevedo; Jussara Pereira do Nascimento; Liliana Cruz Spano
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of medical virology     Volume:  82     ISSN:  1096-9071     ISO Abbreviation:  J. Med. Virol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-09     Completed Date:  2010-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1689-93     Citation Subset:  IM    
Copyright Information:
(c) 2010 Wiley-Liss, Inc.
Center of Infectious Diseases, Health Science Center, Federal University of Espírito Santo, Vitória, ES, Brazil.
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MeSH Terms
Abortion, Spontaneous / etiology*,  virology
Chorion / virology
DNA, Viral / genetics,  isolation & purification
Decidua / virology
Dependovirus / isolation & purification*
In Situ Hybridization / methods
Infant, Newborn
Parvoviridae Infections / complications*,  diagnosis*,  virology
Pathology, Molecular / methods
Polymerase Chain Reaction / methods
Trophoblasts / virology
Virology / methods
Reg. No./Substance:
0/DNA, Viral

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