Document Detail


Molecular detection of Bartonella spp. in the dental pulp of stray cats buried for a year.
MedLine Citation:
PMID:  15652295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bartonella henselae causes chronic bacteremia in cats. To test if B. henselae DNA can be recovered from the dental pulp of cats buried a year previously, we used PCR with primers for a sequence of the conserved groEL to test 104 teeth from 11 cats. Seven of the cats were found positive; canine teeth were more frequently positive than molar teeth. Where PCR sequences could be determined, they were identical to those of B. henselae Marseille (four cats), B. henselae Houston (one cat) or similar to those of B. grahamii (one cat). Our study indicates that dental pulp from the teeth of cats, especially the canine teeth, may be used for the PCR detection of Bartonella in animals buried for a year.
Authors:
Gérard Aboudharam; Dang La Vu; Bernard Davoust; Michel Drancourt; Didier Raoult
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Publication Detail:
Type:  Journal Article     Date:  2004-12-08
Journal Detail:
Title:  Microbial pathogenesis     Volume:  38     ISSN:  0882-4010     ISO Abbreviation:  Microb. Pathog.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-17     Completed Date:  2005-07-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8606191     Medline TA:  Microb Pathog     Country:  England    
Other Details:
Languages:  eng     Pagination:  47-51     Citation Subset:  IM    
Affiliation:
Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerranée, 27, Boulevard Jean Moulin, Marseille Cedex 05 13385, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / genetics
Bartonella / genetics,  isolation & purification*
Bartonella Infections / microbiology,  veterinary*
Bartonella henselae / genetics,  isolation & purification
Burial
Cat Diseases / microbiology*
Cats
Chaperonin 60 / genetics
Cuspid / microbiology
DNA, Bacterial / analysis
Dental Pulp / microbiology*
Exhumation
Genes, Bacterial
Molar / microbiology
Polymerase Chain Reaction
Sequence Analysis, DNA
Time Factors
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Chaperonin 60; 0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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