Document Detail


Molecular cytogenetic evaluation of chromosome instability in Triticum aestivum-Secale cereale disomic addition lines.
MedLine Citation:
PMID:  20453302     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The genetic stability of wheat/rye ('Chinese Spring'/'Imperial') disomic addition lines was checked using the Feulgen method and fluorescent in situ hybridization (FISH). Feulgen staining detected varying proportions of disomic, monosomic, and telosomic plants among the progenies of the disomic addition lines. The greatest stability was observed for the 7R addition line, while the most unstable lines were those with 2R and 4R additions. Chromosome rearrangements were also detected using FISH. Based on the specific hybridization patterns of repetitive DNA probes pSc119.2 and (AAC)(5), as well as ribosomal DNA probes (5S and 45S), isochromosomes were identified in the progenies of 1R and 4R addition lines. The results draw attention to the importance of continuous cytological checks on basic genetic materials by using FISH, because this method reveals chromosome rearrangements that could not be detected either with the conventional Feulgen staining technique or with molecular markers.
Authors:
E Szakács; M Molnár-Láng
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied genetics     Volume:  51     ISSN:  1234-1983     ISO Abbreviation:  J. Appl. Genet.     Publication Date:  2010  
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-08-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9514582     Medline TA:  J Appl Genet     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  149-52     Citation Subset:  IM    
Affiliation:
Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, Hungary.
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MeSH Terms
Descriptor/Qualifier:
Chromosomal Instability*
Cytogenetic Analysis
DNA, Plant / genetics
DNA, Ribosomal / genetics
Gene Frequency
Gene Rearrangement
Hybridization, Genetic
In Situ Hybridization, Fluorescence
Nondisjunction, Genetic
Secale cereale / genetics*
Triticum / genetics*
Uniparental Disomy / genetics
Chemical
Reg. No./Substance:
0/DNA, Plant; 0/DNA, Ribosomal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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