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Molecular cytogenetic analysis of feline leukemia virus insertions in cat lymphoid tumor cells.
MedLine Citation:
PMID:  19879301     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was conducted to map the acquired proviral insertions in the chromosomal genome of feline lymphoid tumors induced by feline leukemia virus (FeLV). Chromosome specimens of the lymphoid tumor-derived cell lines and normal cat lymphocytes were subjected to fluorescence in situ hybridization and tyramide signal amplification, using an exogenous FeLV-A genome as a probe. Specific hybridization signals were detected only on the metaphase chromosomes of the tumor cells. Poisson's distribution-based statistics indicated that 6 chromosomal loci in each cell line showed FeLV integration. In the examination of metaphase chromosomes of FL-74, FT-1 and KO-1 cells, significant signals were detected on B2p15-p14, B2q11, D1p14, E1p14-p13, E1q12 and F2q16; A2p23-p22, B2p15-p14, B4p15-p14, D4q23-q24, E1p14-p13 and E2p13-p12; and A2p22, A3q22, B1p13, B1q13, D1p13 and D3p15-p14, respectively. Consistently, Southern blot hybridization using an FeLV LTR-U3 probe specific for exogenous FeLV revealed the presence of at least 6 copies of exogenous FeLV proviruses at different integration sites in each cell line. These results indicate that there may be common FeLV integration sites at least in A2p22 and B2p15-p14. The cytogenetic analysis used in this study can promptly screen FeLV insertions and provide tags for identifying the novel common integration site.
Authors:
Yasuhito Fujino; Hitoshi Satoh; Koichi Ohno; Hajime Tsujimoto
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-29
Journal Detail:
Title:  Journal of virological methods     Volume:  163     ISSN:  1879-0984     ISO Abbreviation:  J. Virol. Methods     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-02-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  344-52     Citation Subset:  IM    
Copyright Information:
2009 Elsevier B.V. All rights reserved.
Affiliation:
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. afujino@mail.ecc.u-tokyo.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Southern
Cats
Cell Line, Tumor
Chromosomes / virology
Cytogenetic Analysis*
In Situ Hybridization, Fluorescence / methods
Karyotyping
Leukemia Virus, Feline / genetics*
Lymphoma / virology*
Mutagenesis, Insertional
Proviruses / genetics*
Virus Integration*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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