Document Detail

Molecular cytogenetic analysis of feline leukemia virus insertions in cat lymphoid tumor cells.
MedLine Citation:
PMID:  19879301     Owner:  NLM     Status:  MEDLINE    
This study was conducted to map the acquired proviral insertions in the chromosomal genome of feline lymphoid tumors induced by feline leukemia virus (FeLV). Chromosome specimens of the lymphoid tumor-derived cell lines and normal cat lymphocytes were subjected to fluorescence in situ hybridization and tyramide signal amplification, using an exogenous FeLV-A genome as a probe. Specific hybridization signals were detected only on the metaphase chromosomes of the tumor cells. Poisson's distribution-based statistics indicated that 6 chromosomal loci in each cell line showed FeLV integration. In the examination of metaphase chromosomes of FL-74, FT-1 and KO-1 cells, significant signals were detected on B2p15-p14, B2q11, D1p14, E1p14-p13, E1q12 and F2q16; A2p23-p22, B2p15-p14, B4p15-p14, D4q23-q24, E1p14-p13 and E2p13-p12; and A2p22, A3q22, B1p13, B1q13, D1p13 and D3p15-p14, respectively. Consistently, Southern blot hybridization using an FeLV LTR-U3 probe specific for exogenous FeLV revealed the presence of at least 6 copies of exogenous FeLV proviruses at different integration sites in each cell line. These results indicate that there may be common FeLV integration sites at least in A2p22 and B2p15-p14. The cytogenetic analysis used in this study can promptly screen FeLV insertions and provide tags for identifying the novel common integration site.
Yasuhito Fujino; Hitoshi Satoh; Koichi Ohno; Hajime Tsujimoto
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-29
Journal Detail:
Title:  Journal of virological methods     Volume:  163     ISSN:  1879-0984     ISO Abbreviation:  J. Virol. Methods     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-02-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  344-52     Citation Subset:  IM    
Copyright Information:
2009 Elsevier B.V. All rights reserved.
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blotting, Southern
Cell Line, Tumor
Chromosomes / virology
Cytogenetic Analysis*
In Situ Hybridization, Fluorescence / methods
Leukemia Virus, Feline / genetics*
Lymphoma / virology*
Mutagenesis, Insertional
Proviruses / genetics*
Virus Integration*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  An enzymatic virus-like particle assay for sensitive detection of virus entry.
Next Document:  Evaluation of study design variables and their impact on food-maintained operant responding in mice.