| Molecular crowding effects on structure and stability of DNA. | |
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MedLine Citation:
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PMID: 18331845 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Living cells contain a variety of biomolecules including nucleic acids, proteins, polysaccharides, and metabolites as well as other soluble and insoluble components. These biomolecules occupy a significant fraction (20-40%) of the cellular volume. The total concentration of biomolecules reaches 400gL(-1), leading to a crowded intracellular environment referred to as molecular crowding. Therefore, an understanding of the effects of molecular crowding conditions on biomolecules is important to broad research fields such as biochemical, medical, and pharmaceutical sciences. In this review, we describe molecular conditions in the cytoplasm and nucleus, which are totally different from in vitro conditions, and then show the biochemical and biophysical consequences of molecular crowding. Finally, we discuss the effect of molecular crowding on the structure, stability, and function of nucleic acids and the significance of molecular crowding in biotechnology and nanotechnology. |
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Authors:
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Daisuke Miyoshi; Naoki Sugimoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2008-02-21 |
Journal Detail:
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Title: Biochimie Volume: 90 ISSN: 0300-9084 ISO Abbreviation: Biochimie Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-30 Completed Date: 2008-10-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: France |
Other Details:
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Languages: eng Pagination: 1040-51 Citation Subset: IM |
Affiliation:
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Frontier Institute for Biomolecular Engineering Research , Konan University, Kobe 658-8501, Japan. miyoshi@center.konan-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Binding Sites Cell Size DNA / chemistry* Models, Biological* Models, Molecular Nanotechnology Nucleic Acid Conformation Proteins / chemistry Thermodynamics |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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