Document Detail

Molecular composition of biliary phosphatidylcholines, as related to cholesterol saturation, transport and nucleation in human gallbladder bile.
MedLine Citation:
PMID:  1506657     Owner:  NLM     Status:  MEDLINE    
It has been suggested that qualitative changes in bile phosphatidylcholine (lecithin) play a role in the pathogenesis of cholesterol gallstones. We investigated the possible relationship between the molecular composition and hydrophobicity of biliary lecithins and bile cholesterol saturation, nucleation time and the mode of cholesterol transport in human gallbladder bile. Nineteen patients (12 with and seven without gallstones) undergoing abdominal surgery were studied. Bile cholesterol saturation ranged from 77% to 186% (median: 123%) and nucleation time from 1 to 24 days (median: 3 days). Biliary lipid carriers (vesicles and mixed micelles) were separated using Superose-6 gel chromatography and their lipid content was quantitated. Biliary lecithin composition was analyzed by HPLC. Fourteen individual molecular species were detected in the bile: none were related to cholesterol saturation or nucleation time. An arbitrary cumulative index of lecithin hydrophobicity was computed for each bile sample, based on the HPLC capacity factor of the individual species and their percent mole fraction: this index ranged from 47.0 and 58.5 (median: 49) and was unrelated to cholesterol saturation and nucleation time. The biliary concentration of sn-1 palmitoyl:sn-2 arachidonoyl lecithin was significantly correlated (p less than 0.01) with the fraction of cholesterol carried by mixed micelles. This finding suggests that arachidonoyl lecithin may play a modulatory role in the partitioning of cholesterol among biliary carriers. We conclude that major abnormalities in the composition of biliary lecithins are unlikely to play a causative role in the pathogenesis of cholesterol gallstone, although the role of arachidonoyl species requires further investigation.
M Angelico; S Ginanni Corradini; R Masella; D Alvaro; A Cantafora; L Capocaccia
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of hepatology     Volume:  15     ISSN:  0168-8278     ISO Abbreviation:  J. Hepatol.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-09-22     Completed Date:  1992-09-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  59-66     Citation Subset:  IM    
2nd Gastroenterology Division, University of Rome La Sapienza, School of Medicine, Italy.
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MeSH Terms
Bile / chemistry,  metabolism*
Biological Transport
Cholelithiasis / chemistry,  etiology,  physiopathology
Cholesterol / analysis*,  metabolism,  physiology
Chromatography, High Pressure Liquid
Gallbladder / metabolism*
Middle Aged
Phosphatidylcholines / analysis*,  metabolism,  physiology
Reg. No./Substance:
0/Micelles; 0/Phosphatidylcholines; 57-88-5/Cholesterol

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