Document Detail


Molecular composition of biliary phosphatidylcholines, as related to cholesterol saturation, transport and nucleation in human gallbladder bile.
MedLine Citation:
PMID:  1506657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been suggested that qualitative changes in bile phosphatidylcholine (lecithin) play a role in the pathogenesis of cholesterol gallstones. We investigated the possible relationship between the molecular composition and hydrophobicity of biliary lecithins and bile cholesterol saturation, nucleation time and the mode of cholesterol transport in human gallbladder bile. Nineteen patients (12 with and seven without gallstones) undergoing abdominal surgery were studied. Bile cholesterol saturation ranged from 77% to 186% (median: 123%) and nucleation time from 1 to 24 days (median: 3 days). Biliary lipid carriers (vesicles and mixed micelles) were separated using Superose-6 gel chromatography and their lipid content was quantitated. Biliary lecithin composition was analyzed by HPLC. Fourteen individual molecular species were detected in the bile: none were related to cholesterol saturation or nucleation time. An arbitrary cumulative index of lecithin hydrophobicity was computed for each bile sample, based on the HPLC capacity factor of the individual species and their percent mole fraction: this index ranged from 47.0 and 58.5 (median: 49) and was unrelated to cholesterol saturation and nucleation time. The biliary concentration of sn-1 palmitoyl:sn-2 arachidonoyl lecithin was significantly correlated (p less than 0.01) with the fraction of cholesterol carried by mixed micelles. This finding suggests that arachidonoyl lecithin may play a modulatory role in the partitioning of cholesterol among biliary carriers. We conclude that major abnormalities in the composition of biliary lecithins are unlikely to play a causative role in the pathogenesis of cholesterol gallstone, although the role of arachidonoyl species requires further investigation.
Authors:
M Angelico; S Ginanni Corradini; R Masella; D Alvaro; A Cantafora; L Capocaccia
Related Documents :
2210647 - Gallbladder mucin as a pronucleating agent for cholesterol monohydrate crystals in bile.
22315157 - Time sequence of the intensification of the liver glucose production induced by high-fa...
11682027 - Phenotypic characterization of lith genes that determine susceptibility to cholesterol ...
319647 - Effect of citrus pectin on blood lipids and fecal steroid excretion in man.
21943297 - Drinking carrot juice increases total antioxidant status and decreases lipid peroxidati...
19329177 - Reduction in c-reactive protein and ldl cholesterol and cardiovascular event rates afte...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of hepatology     Volume:  15     ISSN:  0168-8278     ISO Abbreviation:  J. Hepatol.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-09-22     Completed Date:  1992-09-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  59-66     Citation Subset:  IM    
Affiliation:
2nd Gastroenterology Division, University of Rome La Sapienza, School of Medicine, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Bile / chemistry,  metabolism*
Biological Transport
Cholelithiasis / chemistry,  etiology,  physiopathology
Cholesterol / analysis*,  metabolism,  physiology
Chromatography, High Pressure Liquid
Female
Gallbladder / metabolism*
Humans
Male
Micelles
Middle Aged
Phosphatidylcholines / analysis*,  metabolism,  physiology
Chemical
Reg. No./Substance:
0/Micelles; 0/Phosphatidylcholines; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hemodynamic effects of a combination of vasopressin and ketanserin in patients with hepatitis b-rela...
Next Document:  Bromosulfophthalein disposition in chronically bile duct obstructed rats.