| Molecular combing. | |
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MedLine Citation:
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PMID: 18770738 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This unit describes an important advance in fiber-FISH technology called molecular combing, in which single DNA molecules are bound by one or both ends to a surface and stretched in a uniform and parallel manner by a receding meniscus. This technique is gentle on the molecules, rapid, and easy to perform. Reliable, quantitative information for genome-wide studies can be obtained without the need for other techniques and a large number of accurate measurements can be made in a single experiment. The authors provide detailed protocols for basic molecular combing, high-resolution physical mapping, and gene-dosage approaches as well as support protocols outlining surface preparation, DNA solution preparation, and probe labeling. |
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Authors:
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C Conti; S Caburet; C Schurra; A Bensimon |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] Volume: Chapter 8 ISSN: 1934-9300 ISO Abbreviation: Curr Protoc Cytom Publication Date: 2001 May |
Date Detail:
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Created Date: 2008-09-04 Completed Date: 2008-09-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100899351 Medline TA: Curr Protoc Cytom Country: United States |
Other Details:
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Languages: eng Pagination: Unit 8.10 Citation Subset: IM |
Affiliation:
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Institut Pasteur, Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA / analysis, genetics DNA Probes / analysis Fluorescent Dyes / analysis, pharmacology Genetic Techniques Humans Hydrogen-Ion Concentration In Situ Hybridization, Fluorescence / methods* Oligonucleotide Probes / chemistry |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 0/Fluorescent Dyes; 0/Oligonucleotide Probes; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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