Document Detail

Molecular cloning and tissue expression patterns of a small conductance calcium-activated potassium channel gene in turbot (Scophthalmus maximus L.).
MedLine Citation:
PMID:  19481607     Owner:  NLM     Status:  MEDLINE    
Calcium-activated potassium channels on plasma membrane enable potassium influx into the cell with ensuing changes in plasma membrane potential and consequent effects on cellular metabolic functions. Recently, this potassium channel was reported to regulate the cellular responses of mammalian immune cells. We have postulated the presence of such a channel in fish immune cells and its potential role in immunoregulation in fish. Employing specific primers and RNA template, we cloned a segment of a novel gene from turbot blood sample and subsequently obtained a full cDNA sequence using RACE approaches. Bioinformatic analysis revealed structural and phylogenetic characteristics of a novel small conductance calcium-activated potassium channel gene, we called TSKCa, which exhibits homologous domains to other species particularly in the transmembrane regions. Full-length TSKCa cDNA is 1698 bp with a 1632 bp open reading frame encoding a protein of 544 amino acids. TSKCa gene is expressed in majority of the tested organs and tissues of turbot. To assess the postulated immune function of TSKCa, we infected turbot with the pathogen Vibrio anguillarum. Here, semi-quantitative RT-PCR analysis demonstrated increased mRNA expression of TSKCa in head kidney, spleen and blood, indicating an important role of TSKCa in these organ tissues that mediate the immune defense response of turbot. In contrast, there was less change in expression in the turbot intestines and liver which were less implicated in the immune response in present study.
Bailin Cong; Guang Han; Xiao-Hang Huang; Sheng-Hao Liu; Chen-Lin Liu; Xue-Zheng Lin; Pei-Qing He; Herbert Gasaino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-28
Journal Detail:
Title:  Fish & shellfish immunology     Volume:  27     ISSN:  1095-9947     ISO Abbreviation:  Fish Shellfish Immunol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-21     Completed Date:  2009-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9505220     Medline TA:  Fish Shellfish Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  221-9     Citation Subset:  IM    
The Key Laboratory of Marine Bioactive Substance, SOA, Qingdao 266061, PR China.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Fish Diseases / immunology
Flatfishes / genetics*,  metabolism*
Gene Expression Profiling
Gene Expression Regulation*
Molecular Sequence Data
RNA, Messenger / metabolism
Sequence Alignment
Small-Conductance Calcium-Activated Potassium Channels / genetics*,  metabolism*
Vibrio / physiology
Vibrio Infections / immunology
Reg. No./Substance:
0/RNA, Messenger; 0/Small-Conductance Calcium-Activated Potassium Channels

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