| [Molecular cloning, sequence analysis and expression of ayu complement component C9 gene]. | |
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MedLine Citation:
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PMID: 22467389 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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C9, a component of the membrane attack complex, participates in the final stage of the complement cascade which lyses foreign organisms by disrupting the integrity of their cell membranes. In the present study, a full-length ayu C9 (aC9) cDNA was cloned which contains 2,125 nucleotides and encodes a protein of 592 amino acids. A signal peptide was deposited in the N-terminal 22 residues. The deduced amino acid sequence of aC9 showed 56.8% identity to the C9 of rainbow trout, and 40.9% to 53.8% identity to the C9 of other teleosts. RT-PCR analysis demonstrated that the mRNA of aC9 was expressed in the liver, spleen, intestine, gill and muscle of healthy ayu fish with the highest level in the liver. Quantitative RT-PCR analysis showed that aC9 transcripts were significantly up-regulated in the liver at 4 h post Listonella anguillarum infection, peaked at 16 h post injection. Western blotting analysis revealed that serum aC9 significantly increased in Listonella anguillarum infected ayu fish. Our results suggested that aC9 may play an important role in fish immune response of anti-bacteria. |
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Authors:
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Cheng-Jiang Kong; Zuo-An Huang; Jiong Chen; Yu-Hong Shi; Xin-Jiang Lu |
Publication Detail:
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Type: English Abstract; Journal Article |
Journal Detail:
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Title: Dong wu xue yan jiu = Zoological research / "Dong wu xue yan jiu" bian ji wei yuan hui bian ji Volume: 33 ISSN: 0254-5853 ISO Abbreviation: Zool. Res. Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-04-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100961499 Medline TA: Dongwuxue Yanjiu Country: China |
Other Details:
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Languages: chi Pagination: 151-7 Citation Subset: IM |
Affiliation:
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Key laboratory of Applied marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China. jchen1975@163.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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