| Molecular cloning of a mercurial-insensitive water channel expressed in selected water-transporting tissues. | |
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MedLine Citation:
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PMID: 7509789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two mercurial-inhibitable water-transporting proteins have been identified: CHIP28, an erythrocyte water channel also expressed in kidney tubules and selected extrarenal epithelia, and WCH-CD, a kidney collecting duct water channel. In searching for a protein responsible for the high transcellular water permeability in lung alveolus, we cloned a 32-kDa water channel (mercurial-insensitive water channel (MIWC)) from a rat lung cDNA library with several novel features. Water permeability was strongly increased in Xenopus oocytes expressing MIWC in a mercurial-insensitive manner, in contrast to known water channels. By in situ hybridization, MIWC showed an unique distribution in cells that do not express CHIP28, including kidney papillary vasa recta, cells lining the subarachnoid space and ventricles in brain, the inner nuclear layer in retina, and the conjunctival epithelium. An alternatively spliced form of MIWC with a 165-base pair deletion in the coding sequence was also identified; relative expression of the spliced mRNA was tissue-specific. The MIWC water channel may participate in the urinary concentrating mechanism, the absorption of cerebrospinal fluid, and other physiological processes. |
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Authors:
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H Hasegawa; T Ma; W Skach; M A Matthay; A S Verkman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 269 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-04-01 Completed Date: 1994-04-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5497-500 Citation Subset: IM |
Affiliation:
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Department of Medicine and Physiology, University of California, San Francisco 94143-0532. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/L27588 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Aquaporin 1 Aquaporins* Base Sequence Biological Transport Cloning, Molecular DNA, Complementary Ion Channels / genetics* Lung / metabolism Membrane Proteins / genetics* Molecular Sequence Data RNA, Messenger / metabolism Rats Sequence Homology, Amino Acid Water / metabolism* Xenopus |
| Grant Support | |
ID/Acronym/Agency:
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DK35124/DK/NIDDK NIH HHS; HL42368/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aqp1 protein, rat; 0/Aquaporins; 0/DNA, Complementary; 0/Ion Channels; 0/Membrane Proteins; 0/RNA, Messenger; 0/mercurial-insensitive water channel; 146410-94-8/Aquaporin 1; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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