Document Detail

Molecular cloning of human squamous cell carcinoma antigen 1 gene and characterization of its promoter.
MedLine Citation:
PMID:  11267667     Owner:  NLM     Status:  MEDLINE    
The squamous cell carcinoma antigen (SCCA) serves as a serological marker for squamous cell carcinomas. Molecular cloning of the SCCA genomic region has revealed the presence of two tandemly arrayed genes, SCCA1 and SCCA2, which are 95% identical in nucleotide sequence. SCCA1 is a papain-like cysteine proteinase inhibitor, while SCCA2 is a chymotrypsin-like serine proteinase inhibitor. We analyzed here the sequence and the promoter activity of the 5'-flanking region of the SCCA1 gene. Deletion analysis of SCCA1 and SCCA2 promoter identified a 471-bp core promoter region upstream of the transcription start site. The transcriptional activity of SCCA1 promoter was up-regulated in squamous cell carcinoma cells, compared with keratinocyte and adenocarcinoma cells. The ratios of SCCA1 to SCCA2 promoter activity in squamous cell carcinoma, keratinocyte and adenocarcinoma cells were respectively 1.6, 5.3 and 2.8. Position -50 of SCCA1 and SCCA2 promoters played an important role in determining the promoter activities of SCCA1 and SCCA2. These findings suggest that the transcriptional regulation of SCCA1 and SCCA2 might differ among squamous cell carcinoma, keratinocyte and adenocarcinoma cells, and that SCCA1 promoter might be a potential target of gene therapy for squamous cell carcinoma.
K Hamada; H Shinomiya; Y Asano; T Kihana; M Iwamoto; Y Hanakawa; K Hashimoto; S Hirose; S Kyo; M Ito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1518     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-27     Completed Date:  2001-06-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  124-31     Citation Subset:  IM    
Department of Obstetrics and Gynecology, School of Medicine, Ehime University, Shitsukawa, Shigenobu, Osen-gun, Ehime 791-0295, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB034984;  AB035089
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MeSH Terms
Antigens, Neoplasm / genetics*
Base Sequence
Carcinoma, Squamous Cell / immunology*
Cloning, Molecular
Molecular Sequence Data
Promoter Regions, Genetic*
Transcriptional Activation
Tumor Cells, Cultured
Uterine Cervical Neoplasms / immunology*
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Serpins; 0/squamous cell carcinoma-related antigen

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