| Molecular cloning of a human cDNA encoding beta-1,4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc beta-1,4-galactosyltransferase. | |
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MedLine Citation:
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PMID: 9435216 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A cDNA encoding a beta-1,4-galactosyltransferase named beta-1,4-GalT II was cloned from a cDNA library of the human breast tumor cell line, MRK-nu-1. Initially, a 860-bp PCR fragment was obtained from MRK-nu-1 mRNA by 3'-rapid amplification of cDNA ends by using two nested degenerate oligonucleotide primers based on a highly conserved amino acid sequence found in the catalytic domain of mammalian beta-1,4-galactosyltransferases and Lymnaea stagnalis beta-1,4-N-acetylglucosaminyltransferase (beta-1,4-GlcNAcT), both of which utilize the same sugar acceptor. This subsequently was used as a probe to isolate a 4.7-kb cDNA that contained an ORF of 1,164 bp predicting a polypeptide of 388 aa. Its deduced amino acid sequence shows an identity of 37% with that of the previously characterized human beta-1,4-galactosyltransferase (referred to as beta-1,4-GalT I) and of 28% with that of L. stagnalis beta-1,4-GlcNAcT. Study of the properties of the beta-1,4-GalT II fused to protein A expressed as a soluble form in COS-7 cells revealed that it is a genuine beta-1,4-GalT but has no lactose synthetase activity in the presence of alpha-lactalbumin. Northern blot analysis of 24 human tissues showed that they all express the beta-1,4-GalT II transcript, although the levels varied. These results indicate that human cells contain another beta-1,4-GalT. |
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Authors:
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T Sato; K Furukawa; H Bakker; D H Van den Eijnden; I Van Die |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 95 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1998 Jan |
Date Detail:
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Created Date: 1998-02-24 Completed Date: 1998-02-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 472-7 Citation Subset: IM |
Affiliation:
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Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB004550 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Complementary / genetics*, isolation & purification Humans Molecular Sequence Data Sequence Homology, Nucleic Acid beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase / genetics* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; EC 2.4.1.38/beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase |
| Comments/Corrections | |
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