| Molecular cloning of goat 20alpha-hydroxysteroid dehydrogenase cDNA. | |
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MedLine Citation:
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PMID: 15226597 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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20Alpha-hydroxysteroid dehydrogenase (20alpha-HSD), which catalyzes the conversion of progesterone to its inactive form 20alpha-dihydroprogesterone, is expressed in murine placenta and has been suggested to play roles in maintaining pregnancy. To understand the role of 20alpha-HSD during pregnancy in the goat, as a first step, cloning and sequencing of 20alpha-HSD cDNA were performed. The full nucleotide sequence of 20alpha-HSD cDNA was determined on samples obtained from the corpus luteum at the luteal phase of the estrous cycle and the placenta in late pregnancy by RT-PCR and 3' and 5' RACE systems. Cloned 20alpha-HSD cDNA consisted of 1124 bp and belonged to the aldo-keto reductase superfamily. From the start codon to stop codon there were 323 amino acids, the same as in other species. To verify whether the protein derived from goat 20alpha-HSD cDNA had 20alpha-HSD activity, the cDNA was expressed by bacteria. Bacterially expressed goat 20alpha-HSD protein showed 20alpha-HSD enzyme activity. A tissue distribution study demonstrated that 20alpha-HSD was expressed in the placenta, but not in the adrenal gland, liver and spleen during pregnancy. The present study suggests that goat 20alpha-HSD is another member of the aldo-keto reductase superfamily and that it plays a role in the placenta during pregnancy. |
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Authors:
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Walimuni Samantha Nilanthi Jayasekara; Tomohiro Yonezawa; Maho Ishida; Keitaro Yamanouchi; Masugi Nishihara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of reproduction and development Volume: 50 ISSN: 0916-8818 ISO Abbreviation: J. Reprod. Dev. Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-06-30 Completed Date: 2005-01-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9438792 Medline TA: J Reprod Dev Country: Japan |
Other Details:
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Languages: eng Pagination: 323-31 Citation Subset: IM |
Affiliation:
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Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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20-alpha-Hydroxysteroid Dehydrogenase
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biosynthesis*,
genetics* Amino Acid Sequence Animals Base Sequence Blotting, Western Catalysis Cloning, Molecular Codon, Initiator Codon, Terminator Corpus Luteum / metabolism DNA, Complementary / metabolism Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Escherichia coli / metabolism Female Genetic Vectors Glutathione Transferase / metabolism Goats Molecular Sequence Data Ovary / metabolism Placenta / metabolism Pregnancy Pregnancy, Animal Protein Structure, Tertiary RNA, Messenger / metabolism Recombinant Fusion Proteins / metabolism Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Time Factors Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Codon, Initiator; 0/Codon, Terminator; 0/DNA, Complementary; 0/RNA, Messenger; 0/Recombinant Fusion Proteins; 0/Recombinant Proteins; EC 1.1.1.149/20-alpha-Hydroxysteroid Dehydrogenase; EC 2.5.1.18/Glutathione Transferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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