|Molecular cloning of goat 20alpha-hydroxysteroid dehydrogenase cDNA.|
|PMID: 15226597 Owner: NLM Status: MEDLINE|
|20Alpha-hydroxysteroid dehydrogenase (20alpha-HSD), which catalyzes the conversion of progesterone to its inactive form 20alpha-dihydroprogesterone, is expressed in murine placenta and has been suggested to play roles in maintaining pregnancy. To understand the role of 20alpha-HSD during pregnancy in the goat, as a first step, cloning and sequencing of 20alpha-HSD cDNA were performed. The full nucleotide sequence of 20alpha-HSD cDNA was determined on samples obtained from the corpus luteum at the luteal phase of the estrous cycle and the placenta in late pregnancy by RT-PCR and 3' and 5' RACE systems. Cloned 20alpha-HSD cDNA consisted of 1124 bp and belonged to the aldo-keto reductase superfamily. From the start codon to stop codon there were 323 amino acids, the same as in other species. To verify whether the protein derived from goat 20alpha-HSD cDNA had 20alpha-HSD activity, the cDNA was expressed by bacteria. Bacterially expressed goat 20alpha-HSD protein showed 20alpha-HSD enzyme activity. A tissue distribution study demonstrated that 20alpha-HSD was expressed in the placenta, but not in the adrenal gland, liver and spleen during pregnancy. The present study suggests that goat 20alpha-HSD is another member of the aldo-keto reductase superfamily and that it plays a role in the placenta during pregnancy.|
|Walimuni Samantha Nilanthi Jayasekara; Tomohiro Yonezawa; Maho Ishida; Keitaro Yamanouchi; Masugi Nishihara|
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|Type: Journal Article; Research Support, Non-U.S. Gov't|
|Title: The Journal of reproduction and development Volume: 50 ISSN: 0916-8818 ISO Abbreviation: J. Reprod. Dev. Publication Date: 2004 Jun|
|Created Date: 2004-06-30 Completed Date: 2005-01-06 Revised Date: 2006-11-15|
Medline Journal Info:
|Nlm Unique ID: 9438792 Medline TA: J Reprod Dev Country: Japan|
|Languages: eng Pagination: 323-31 Citation Subset: IM|
|Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo, Japan.|
|APA/MLA Format Download EndNote Download BibTex|
Amino Acid Sequence
Corpus Luteum / metabolism
DNA, Complementary / metabolism
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Escherichia coli / metabolism
Glutathione Transferase / metabolism
Molecular Sequence Data
Ovary / metabolism
Placenta / metabolism
Protein Structure, Tertiary
RNA, Messenger / metabolism
Recombinant Fusion Proteins / metabolism
Recombinant Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
|0/Codon, Initiator; 0/Codon, Terminator; 0/DNA, Complementary; 0/RNA, Messenger; 0/Recombinant Fusion Proteins; 0/Recombinant Proteins; EC 220.127.116.11/20-alpha-Hydroxysteroid Dehydrogenase; EC 18.104.22.168/Glutathione Transferase|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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