Document Detail


Molecular cloning of gene sequences from rat heart rapidly responsive to pressure overload.
MedLine Citation:
PMID:  2317898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although pressure overload induces cardiac hypertrophy and reexpression of contractile protein isogenes, the molecular mechanism of both is not well understood. We demonstrated the early change in the translational activity of specific cardiac messenger RNA by two-dimensional gel electrophoresis of in vitro translational products. A total of over 400 translational products were detected by fluorography, and the relative predominance of eight species was increased by pressure overload whereas that of two translational products was decreased. We cloned four pressure-overload-responsive complementary DNA clones, pPO-1, -4, -5, and -6, by differential dot blot hybridization. Two clones were increased from the early period after the imposition of pressure overload; the other two were decreased. The expression pattern of each complementary DNA clone in the pressure-overloaded hearts was similar to that in fetal hearts. Pressure overload also induced the additional messenger RNA, which hybridized with pPO-4. This mRNA was also recognized in fetal, neonatal, and adult spontaneously hypertensive rat hearts. The induction of this transcript by pressure overload was not suppressed but, rather, stimulated by cycloheximide. These results suggest that there are some genes that rapidly respond to pressure overload and that may play some role in the development of cardiac hypertrophy.
Authors:
I Komuro; Y Shibazaki; M Kurabayashi; F Takaku; Y Yazaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Circulation research     Volume:  66     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-05-09     Completed Date:  1990-05-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  979-85     Citation Subset:  IM    
Affiliation:
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Animals
Animals, Newborn
Blotting, Northern
Cells, Cultured
Cloning, Molecular*
Cycloheximide / pharmacology
Embryo, Mammalian / physiology
Gene Expression Regulation
Genes*
Heart / physiopathology*
Hypertension / genetics*,  physiopathology
Male
Protein Biosynthesis
RNA, Messenger
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/RNA, Messenger; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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