| Molecular cloning of gene sequences from rat heart rapidly responsive to pressure overload. | |
| | |
MedLine Citation:
|
PMID: 2317898 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Although pressure overload induces cardiac hypertrophy and reexpression of contractile protein isogenes, the molecular mechanism of both is not well understood. We demonstrated the early change in the translational activity of specific cardiac messenger RNA by two-dimensional gel electrophoresis of in vitro translational products. A total of over 400 translational products were detected by fluorography, and the relative predominance of eight species was increased by pressure overload whereas that of two translational products was decreased. We cloned four pressure-overload-responsive complementary DNA clones, pPO-1, -4, -5, and -6, by differential dot blot hybridization. Two clones were increased from the early period after the imposition of pressure overload; the other two were decreased. The expression pattern of each complementary DNA clone in the pressure-overloaded hearts was similar to that in fetal hearts. Pressure overload also induced the additional messenger RNA, which hybridized with pPO-4. This mRNA was also recognized in fetal, neonatal, and adult spontaneously hypertensive rat hearts. The induction of this transcript by pressure overload was not suppressed but, rather, stimulated by cycloheximide. These results suggest that there are some genes that rapidly respond to pressure overload and that may play some role in the development of cardiac hypertrophy. |
| | |
Authors:
|
I Komuro; Y Shibazaki; M Kurabayashi; F Takaku; Y Yazaki |
Related Documents
:
|
16189198 - Regulation of myocardial glucose transporters glut1 and glut4 in chronically anemic fet... 8498228 - Sonography of the fetal heart: normal variants and pitfalls. 17943858 - Fundal pressure versus controlled cord traction as part of the active management of the... 12459688 - Fetal and maternal effects of phenylephrine and ephedrine during spinal anesthesia for ... 20226988 - Prognostic significance of visit-to-visit variability, maximum systolic blood pressure,... 14676378 - Experiment of seed germination with low pressure environment simulator. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Circulation research Volume: 66 ISSN: 0009-7330 ISO Abbreviation: Circ. Res. Publication Date: 1990 Apr |
Date Detail:
|
Created Date: 1990-05-09 Completed Date: 1990-05-09 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0047103 Medline TA: Circ Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 979-85 Citation Subset: IM |
Affiliation:
|
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acute Disease Animals Animals, Newborn Blotting, Northern Cells, Cultured Cloning, Molecular* Cycloheximide / pharmacology Embryo, Mammalian / physiology Gene Expression Regulation Genes* Heart / physiopathology* Hypertension / genetics*, physiopathology Male Protein Biosynthesis RNA, Messenger Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Messenger; 66-81-9/Cycloheximide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Collagen and elastin metabolism in hypertensive pulmonary arteries of rats.
Next Document: Decreased contractile efficiency and increased nonmechanical energy cost in hyperthyroid rabbit hear...