Document Detail

Molecular cloning and functional characterization of the mouse mafB gene.
MedLine Citation:
PMID:  10721736     Owner:  NLM     Status:  MEDLINE    
The Maf family of the transcription factors plays a pivotal role in controlling development and cellular differentiation. To clarify the molecular mechanisms controlling mafB expression, a genomic clone of the mouse mafB gene was isolated and analyzed. RNase protection analysis determined the transcription initiation site at 389 bp upstream from the translation initiation site. The 3' end of the gene is located at 946 bp downstream from the termination codon. The gene lacks intron structure. Sequence analysis showed a TATA-like sequence (5'-GATAAAA-3') and an inverted CCAAT-box (5'-ATTGG-3') in the promoter region. Upstream of these sequences, there are several potential regulatory elements, including two GC-boxes (5'-GGGCGG-3'), and a palindromic sequence (5'-GTCAGCTGAC-3') which contains two Maf recognition elements (MARE, 5'-GCTGAC-3') and an E-box (5'-CAGCTG-3'). Transient transfection analysis with the 5'-flanking region of the mafB gene demonstrated that these elements are important for mafB gene expression. In addition, cotransfection analysis indicated that the MyoD activates the mouse mafB promoter and the gene is positively auto-regulated by its own product.
K Huang; M S Serria; H Nakabayashi; S Nishi; M Sakai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  242     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-04-03     Completed Date:  2000-04-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  419-26     Citation Subset:  IM    
Department of Biochemistry, Hokkaido University School of Medicine, Sapporo, Japan.
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MeSH Terms
Avian Proteins*
Base Sequence
Cloning, Molecular
DNA / chemistry,  genetics
DNA-Binding Proteins*
Genes / genetics
Luciferases / genetics,  metabolism
MafB Transcription Factor
Mice, Inbred Strains
Molecular Sequence Data
MyoD Protein / physiology
Oncogene Proteins / genetics*,  physiology
Promoter Regions, Genetic / genetics,  physiology
Recombinant Fusion Proteins / genetics,  metabolism
Sequence Analysis, DNA
Sequence Deletion
Trans-Activators / genetics*,  physiology
Transcription Factors*
Transcription, Genetic
Transcriptional Activation
Tumor Cells, Cultured
Reg. No./Substance:
0/Avian Proteins; 0/DNA-Binding Proteins; 0/MafB Transcription Factor; 0/Mafb protein, mouse; 0/MyoD Protein; 0/Oncogene Proteins; 0/Recombinant Fusion Proteins; 0/Trans-Activators; 0/Transcription Factors; 9007-49-2/DNA; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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