Document Detail


Molecular cloning and functional analysis of a blue light receptor gene MdCRY2 from apple (Malus domestica).
MedLine Citation:
PMID:  23314496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MdCRY2 was isolated from apple fruit skin, and its function was analyzed in MdCRY2 transgenic Arabidopsis. The interaction between MdCRY2 and AtCOP1 was found by yeast two-hybrid and BiFC assays. Cryptochromes are blue/ultraviolet-A (UV-A) light receptors involved in regulating various aspects of plant growth and development. Investigations of the structure and functions of cryptochromes in plants have largely focused on Arabidopsis (Arabidopsis thaliana), tomato (Solanum lycopersicum), pea (Pisum sativum), and rice (Oryza sativa). However, no data on the function of CRY2 are available in woody plants. In this study, we isolated a cryptochrome gene, MdCRY2, from apple (Malus domestica). The deduced amino acid sequences of MdCRY2 contain the conserved N-terminal photolyase-related domain and the flavin adenine dinucleotide (FAD) binding domain, as well as the C-terminal DQXVP-acidic-STAES (DAS) domain. Relationship analysis indicates that MdCRY2 shows the highest similarity to the strawberry FvCRY protein. The expression of MdCRY2 is induced by blue/UV-A light, which represents a 48-h circadian rhythm. To investigate the function of MdCRY2, we overexpressed the MdCRY2 gene in a cry2 mutant and wild type (WT) Arabidopsis, assessed the phenotypes of the resulting transgenic plants, and found that MdCRY2 functions to regulate hypocotyl elongation, root growth, flower initiation, and anthocyanin accumulation. Furthermore, we examined the interaction between MdCRY2 and AtCOP1 using a yeast two-hybrid assay and a bimolecular fluorescence complementation assay. These data provide functional evidence for a role of blue/UV-A light-induced MdCRY2 in controlling photomorphogenesis in apple.
Authors:
Yuan-Yuan Li; Ke Mao; Cheng Zhao; Xian-Yan Zhao; Rui-Fen Zhang; Hua-Lei Zhang; Huai-Rui Shu; Yu-Jin Hao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-13
Journal Detail:
Title:  Plant cell reports     Volume:  32     ISSN:  1432-203X     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-15     Completed Date:  2013-09-18     Revised Date:  2013-11-07    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  555-66     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Arabidopsis / genetics,  growth & development,  metabolism
Arabidopsis Proteins / metabolism
Cloning, Molecular
Cryptochromes / genetics,  metabolism*
Genetic Complementation Test
Light
Malus / genetics*,  growth & development,  metabolism
Molecular Sequence Data
Plant Proteins / genetics,  metabolism*
Plants, Genetically Modified / genetics,  metabolism
Two-Hybrid System Techniques
Ubiquitin-Protein Ligases / metabolism
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Cryptochromes; 0/Plant Proteins; EC 6.3.2.19/COP1 protein, Arabidopsis; EC 6.3.2.19/Ubiquitin-Protein Ligases

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