| Molecular cloning and expression of human carnitine octanoyltransferase: evidence for its role in the peroxisomal beta-oxidation of branched-chain fatty acids. | |
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MedLine Citation:
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PMID: 10486279 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To study the putative role of human carnitine octanoyltransferase (COT) in the beta-oxidation of branched-chain fatty acids, we identified and cloned the cDNA encoding human COT and expressed it in the yeast Saccharomyces cerevisiae. Enzyme activity measurements showed that COT efficiently converts one of the end products of the peroxisomal beta-oxidation of pristanic acid, 4, 8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. Production of the carnitine ester of this branched/medium-chain acyl-CoA within the peroxisome is required for its transport to the mitochondrion where further beta-oxidation occurs. In contrast, 4, 8-dimethylnonanoyl-CoA is not a substrate for carnitine acetyltransferase, another acyltransferase localized in peroxisomes, which catalyzes the formation of carnitine esters of the other products of pristanic acid beta-oxidation, namely acetyl-CoA and propionyl-CoA. Our results shed new light on the function of COT in fatty acid metabolism and point to a crucial role of COT in the beta-oxidation of branched-chain fatty acids. |
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Authors:
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S Ferdinandusse; J Mulders; L IJlst; S Denis; G Dacremont; H R Waterham; R J Wanders |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 263 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-10-21 Completed Date: 1999-10-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 213-8 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Departments of Clinical Chemistry and Pediatrics, Emma Children's Hospital, Academic Medical Center, Amsterdam, 1100 DE, The Netherlands. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF168793 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Carnitine Acyltransferases / genetics*, metabolism* Cattle Cloning, Molecular DNA Primers / genetics DNA, Complementary / genetics Fatty Acids / metabolism* Gene Expression Humans Microbodies / metabolism Molecular Sequence Data Oxidation-Reduction Rats Recombinant Proteins / genetics, metabolism Saccharomyces cerevisiae / genetics Sequence Homology, Amino Acid Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Complementary; 0/Fatty Acids; 0/Recombinant Proteins; 1189-37-3/pristanic acid; EC 2.3.1.-/Carnitine Acyltransferases; EC 2.3.1.-/carnitine octanoyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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