Document Detail


Molecular cloning, expression, and characterization of rat homolog of human AP-2alpha that stimulates neuropeptide Y transcription activity in response to nerve growth factor.
MedLine Citation:
PMID:  10847586     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuropeptide Y (NPY) plays an important role in the central regulation of neuronal activity, endocrine and sexual behavior, and food intake. Although transcription activity of the NPY gene in PC12 cells is regulated by a number of agents such as nerve growth factor (NGF), the mechanism responsible for the NGF-elicited increase in the transcription of the NPY gene remains to be explored. In this study, we isolated and characterized a nuclear protein that is bound to NGF-response elements (NGFRE) that lie between nucleotide -87 and -33 of the rat NPY promoter gene. This nuclear protein is identical to the rat homolog of human transcription factor AP-2alpha. We further demonstrated that rat AP-2a promotes efficient NPY transcription activity in response to NGF. Finally, we provide direct evidence that the mice lacking transcription factor AP-2alpha exhibit reduced expression of NPY mRNA compared with wild-type mice, further supporting the hypothesis that AP-2alpha is an important transcription factor in regulating NPY transcription activity.
Authors:
B S Li; P R Kramer; W Zhao; W Ma; D A Stenger; L Zhang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  14     ISSN:  0888-8809     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-10-03     Completed Date:  2000-10-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  837-47     Citation Subset:  IM    
Affiliation:
Laboratory of Neurochemistry, NINDS, National Institutes of Health Bethesda, Maryland 20892-4130, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Brain Chemistry
Cloning, Molecular*
DNA / metabolism
DNA-Binding Proteins / genetics*,  metabolism,  pharmacology*
Gene Expression / drug effects*
Humans
Mice
Mice, Knockout
Molecular Sequence Data
Nerve Growth Factor / pharmacology*
Neuropeptide Y / genetics*
PC12 Cells
Promoter Regions, Genetic
RNA, Messenger / analysis
Rats
Response Elements
Sequence Homology
Transcription Factor AP-2
Transcription Factors / genetics*,  metabolism,  pharmacology*
Transcription, Genetic / drug effects
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Neuropeptide Y; 0/RNA, Messenger; 0/TFAP2A protein, human; 0/Tcfap2a protein, mouse; 0/Transcription Factor AP-2; 0/Transcription Factors; 9007-49-2/DNA; 9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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