| Molecular cloning and expression of canine hepcidin. | |
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MedLine Citation:
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PMID: 15570559 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Hepcidin is a recently identified acute phase protein with antimicrobial and iron regulatory functions. It has been suggested that hepcidin may be the key mediator of anemia of chronic disease. Our research group is interested in developing a diagnostic assay to measure hepcidin in dogs. OBJECTIVES: The objectives of this study were to clone and sequence the canine hepcidin gene and to gather preliminary data about tissue expression of hepcidin in dogs. METHODS: RNA was extracted from fresh canine liver tissue and cDNA was generated and amplified. Standard reverse transcription polymerase chain reaction techniques were used with degenerate primers based on sequence homology between several other species. The amino acid (AA) sequence was compared with known sequences in other species. Tissue expression of canine hepcidin was determined by Western blot. RESULTS: The canine hepcidin cDNA sequence encoded a highly conserved protein of 85 AAs with 8 cysteine residues at the C-terminal end. This protein was likely the precursor form (pro-hepcidin) of a smaller secreted peptide. Phylogenetic analysis showed that human hepcidin was more homologous with canine than with rodent hepcidin. In dogs, as in people, hepcidin was expressed most strongly in the liver. Western blotting showed a clear band of approximately 9 kDa, consistent with pro-hepcidin. Weak expression was also detected in canine kidney and lung tissues. CONCLUSION: The results of this study establish the basis for future investigation involving canine hepcidin and suggest that the dog may be a suitable model for studying the role of hepcidin in human health and disease. |
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Authors:
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Michael M Fry; Jason L Liggett; Seung Joon Baek |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Veterinary clinical pathology / American Society for Veterinary Clinical Pathology Volume: 33 ISSN: 0275-6382 ISO Abbreviation: Vet Clin Pathol Publication Date: 2004 |
Date Detail:
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Created Date: 2004-11-30 Completed Date: 2005-02-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9880575 Medline TA: Vet Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 223-7 Citation Subset: IM |
Affiliation:
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Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY590589 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antimicrobial Cationic Peptides / analysis, chemistry*, genetics* Base Sequence Cloning, Molecular Dogs / genetics* Molecular Sequence Data Sequence Homology, Amino Acid Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Antimicrobial Cationic Peptides; 0/hepcidin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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