Document Detail

Molecular cloning and expression analysis of a dorsal homologue from Eriocheir sinensis.
MedLine Citation:
PMID:  23981326     Owner:  NLM     Status:  Publisher    
Dorsal as a crucial component of Toll signaling pathway, played important roles in induction and regulation of innate immune responses. In this study, we cloned a NF-κB-like transcription factor Dorsal from Eriocheir sinensis and designated it as EsDorsal. The full-length cDNA of EsDorsal was 2,493 bp with a 2,022-bp open reading frame (ORF) encoding a 673-amino acid protein. This protein contained a 171-residue conserved Rel homology domain (RHD) and a 102-residue Ig-like, plexins and transcription factors domain (IPT). By phylogenetic analysis, EsDorsal was clustered into one group together with other invertebrate Dorsals or NF-κBs, and then clustered with vertebrate NF-κBs. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis results showed that a) EsDorsal had higher expression level in immune organs; b) EsDorsal differentially induced after injection of lipopolysaccharides (LPS), peptidoglycan (PG) or zymosan (GLU). Importantly, EsDorsal was more responsive to LPS than GLU and PG. Collectively, EsDorsal was differentially inducibility in response to various PAMPs, suggesting its involvement in a specific innate immune regulation in E. sinensis.
Ai-Qing Yu; Xing-Kun Jin; Shuang Li; Xiao-Nv Guo; Min-Hao Wu; Wei-Wei Li; Qun Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-8-24
Journal Detail:
Title:  Developmental and comparative immunology     Volume:  -     ISSN:  1879-0089     ISO Abbreviation:  Dev. Comp. Immunol.     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-8-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7708205     Medline TA:  Dev Comp Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013. Published by Elsevier Ltd.
School of Life Science, East China Normal University, Shanghai, China.
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