| Molecular cloning and chromosome localization of a putative basolateral Na(+)-K(+)-2Cl- cotransporter from mouse inner medullary collecting duct (mIMCD-3) cells. | |
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MedLine Citation:
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PMID: 7929272 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electroneutral Na(+)-K(+)-2Cl- cotransporters represent one of the major routes for Cl- movement in epithelia. A secretory form of the cotransporter has been described in the basolateral membrane of a variety of epithelia from fish to mammals. We isolated a putative bumetanide-sensitive Na(+)-K(+)-2Cl- cotransporter cDNA, BSC2, from mIMCD-3 cells. Northern analysis indicates that in contrast to BSC1, the recently cloned renal-specific apical isoform of the cotransporter, BSC2 is expressed in secretory epithelia and thus appears to represent the basolateral isoform. Furthermore, BSC2 is also expressed in non-polarized cells, such as red cells and myocytes. Sequence comparison and chromosome localization demonstrate that BSC2 and BSC1 are different genes that diverged before the evolution of vertebrates. |
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Authors:
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E Delpire; M I Rauchman; D R Beier; S C Hebert; S R Gullans |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 269 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1994 Oct |
Date Detail:
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Created Date: 1994-11-17 Completed Date: 1994-11-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 25677-83 Citation Subset: IM |
Affiliation:
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Harvard Center for Study of Kidney Disease, Renal Division, Harvard Medical School, Boston, Massachusetts. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U13174 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Biological Transport Carrier Proteins / genetics* Cell Line Chlorides / metabolism* Chromosome Mapping Cloning, Molecular Evolution Kidney Medulla / cytology Kidney Tubules, Collecting / cytology Mice Molecular Sequence Data Polymorphism, Genetic Potassium / metabolism* RNA, Messenger / analysis Sodium / metabolism* Sodium-Potassium-Chloride Symporters |
| Grant Support | |
ID/Acronym/Agency:
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DK36031/DK/NIDDK NIH HHS; DK36803/DK/NIDDK NIH HHS; HL49251/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Chlorides; 0/RNA, Messenger; 0/Sodium-Potassium-Chloride Symporters; 7440-09-7/Potassium; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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