Document Detail


Molecular cloning and chromosomal localization of a prophenoloxidase cDNA from the malaria vector Anopheles gambiae.
MedLine Citation:
PMID:  9459428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cDNA clone for prophenoloxidase was isolated from the most important human malaria vector, Anopheles gambiae. The clone encoded a polypeptide of 79341 Da that contains the two copper binding domains common to all invertebrate prophenoloxidases and haemocyanins. Expression of the prophenoloxidase gene was detected throughout all life stages from egg to imago in two strains of A. gambiae; however, the strongest expression was observed in developing embryos in eggs. The prophenoloxidase gene was mapped to the inversion rich region of the right arm of chromosome-2 in region 13B.
Authors:
W J Lee; A Ahmed; A della Torre; A Kobayashi; M Ashida; P T Brey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Insect molecular biology     Volume:  7     ISSN:  0962-1075     ISO Abbreviation:  Insect Mol. Biol.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-12     Completed Date:  1998-03-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9303579     Medline TA:  Insect Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  41-50     Citation Subset:  IM    
Affiliation:
Medical Research Center, College of Medicine, Yonsei University, Seoul, South Korea.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/L76038
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Anopheles / enzymology*,  genetics
Base Sequence
Catechol Oxidase / genetics*
Cats
Chromosome Mapping*
Cloning, Molecular
DNA, Complementary
Enzyme Precursors / genetics*
Gene Expression
Genes, Insect*
Humans
Insect Proteins / genetics
Insect Vectors / enzymology*,  genetics
Malaria / transmission
Molecular Sequence Data
RNA, Messenger
Rabbits
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Enzyme Precursors; 0/Insect Proteins; 0/RNA, Messenger; EC 1.10.3.-/pro-phenoloxidase; EC 1.10.3.1/Catechol Oxidase

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