Document Detail


Molecular cloning and characterization of a vacuolar H+ -pyrophosphatase gene, SsVP, from the halophyte Suaeda salsa and its overexpression increases salt and drought tolerance of Arabidopsis.
MedLine Citation:
PMID:  16463098     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The chenopodiaceae Suaeda salsa L. is a leaf succulent euhalophyte. Shoots of the S. salsa are larger and more succulent when grown in highly saline environments. This increased growth and water uptake has been correlated with a large and specific cellular accumulation of sodium. S. salsa does not have salt glands or salt bladders on its leaves. Thus, this plant must compartmentalize the toxic Na(+) in the vacuoles. The ability to compartmentalize sodium may result from a stimulation of the proton pumps that provide the driving force for increased sodium transport into the vacuole. In this work, we isolated the cDNA of the vacuolar membrane proton-translocating inorganic pyrophosphatase (H(+) -PPase) from S. salsa. The SsVP cDNA contains an uninterrupted open reading frame of 2292 bp, coding for a polypeptide of 764 amino acids. Northern blotting analysis showed that SsVP was induced in salinity treated leaves. The activities of both the V-ATPase and the V-PPase in Arabidopsis overexpressing SsVP-2 is higher markedly than in wild-type plant under 200 mM NaCl and drought stresses. The Overexpression of SsVP can increase salt and drought tolerance of transgenic Arabidopsis.
Authors:
Shanli Guo; Haibo Yin; Xia Zhang; Fengyun Zhao; Pinghua Li; Shihua Chen; Yanxiu Zhao; Hui Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant molecular biology     Volume:  60     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-02-07     Completed Date:  2006-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  41-50     Citation Subset:  IM    
Affiliation:
Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / drug effects
Amino Acid Sequence
Arabidopsis / drug effects*,  genetics,  physiology*
Base Sequence
Chenopodiaceae / enzymology*,  genetics,  growth & development
Cloning, Molecular
Desiccation
Gene Expression
Molecular Sequence Data
Plants, Genetically Modified
Pyrophosphatases / chemistry,  genetics*,  metabolism*
Sodium Chloride / pharmacology*
Water / pharmacology*
Chemical
Reg. No./Substance:
7647-14-5/Sodium Chloride; 7732-18-5/Water; EC 3.6.1.-/Pyrophosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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