| Molecular cloning and characterization of a vacuolar H+ -pyrophosphatase gene, SsVP, from the halophyte Suaeda salsa and its overexpression increases salt and drought tolerance of Arabidopsis. | |
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MedLine Citation:
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PMID: 16463098 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The chenopodiaceae Suaeda salsa L. is a leaf succulent euhalophyte. Shoots of the S. salsa are larger and more succulent when grown in highly saline environments. This increased growth and water uptake has been correlated with a large and specific cellular accumulation of sodium. S. salsa does not have salt glands or salt bladders on its leaves. Thus, this plant must compartmentalize the toxic Na(+) in the vacuoles. The ability to compartmentalize sodium may result from a stimulation of the proton pumps that provide the driving force for increased sodium transport into the vacuole. In this work, we isolated the cDNA of the vacuolar membrane proton-translocating inorganic pyrophosphatase (H(+) -PPase) from S. salsa. The SsVP cDNA contains an uninterrupted open reading frame of 2292 bp, coding for a polypeptide of 764 amino acids. Northern blotting analysis showed that SsVP was induced in salinity treated leaves. The activities of both the V-ATPase and the V-PPase in Arabidopsis overexpressing SsVP-2 is higher markedly than in wild-type plant under 200 mM NaCl and drought stresses. The Overexpression of SsVP can increase salt and drought tolerance of transgenic Arabidopsis. |
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Authors:
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Shanli Guo; Haibo Yin; Xia Zhang; Fengyun Zhao; Pinghua Li; Shihua Chen; Yanxiu Zhao; Hui Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Plant molecular biology Volume: 60 ISSN: 0167-4412 ISO Abbreviation: Plant Mol. Biol. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-02-07 Completed Date: 2006-04-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9106343 Medline TA: Plant Mol Biol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 41-50 Citation Subset: IM |
Affiliation:
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Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological
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drug effects Amino Acid Sequence Arabidopsis / drug effects*, genetics, physiology* Base Sequence Chenopodiaceae / enzymology*, genetics, growth & development Cloning, Molecular Desiccation Gene Expression Molecular Sequence Data Plants, Genetically Modified Pyrophosphatases / chemistry, genetics*, metabolism* Sodium Chloride / pharmacology* Water / pharmacology* |
| Chemical | |
Reg. No./Substance:
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7647-14-5/Sodium Chloride; 7732-18-5/Water; EC 3.6.1.-/Pyrophosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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