Document Detail


Molecular cloning and characterization of a novel rat CXC chemokine, rPBP, the homologue of human and mouse PBP.
MedLine Citation:
PMID:  15016410     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously cloned the mouse platelet basic protein (mPBP), a homologue of human PBP, from mouse thymic stromal cells. Using EST alignment and RT-PCR, the rat homologue of human and mouse PBP was cloned from lung and named as rPBP. The complete open reading frame and part of the 3'- and 5'-non-coding regions were obtained through rapid amplification of cDNA ends. The rPBP cDNA encodes a protein of 111 amino acids containing a signal peptide of 37 amino acids at the N-terminus, with the mature protein of 74 amino acids. The rPBP is a new member of ELR+CXC chemokines. The mature protein of rPBP shares 69% and 45% homology with mouse and human PBP, respectively. In situ hybridization assay revealed rPBP to be predominantly localized in the pulmonary vascular endothelial cells. The eukaryotic expression vector pCDNA3-rPBP was constructed and transiently transfected into COS-7 cells. In the in vitro chemotaxis assay, the polymorphonuclear leukocytes (PMNs) were chemoattracted to the supernatants from transfected COS-7 cells in a dose-dependent manner. The implication of rPBP found in rat lung is that this chemokine may have the function to recruit PMNs to fight against pulmonary infection.
Authors:
Wen-Xian Fu; Meng-Lei Zhu; Shun-You Gong; Yan Li; Wei-Feng Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytokine     Volume:  26     ISSN:  1043-4666     ISO Abbreviation:  Cytokine     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-12     Completed Date:  2004-12-10     Revised Date:  2007-08-16    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37-43     Citation Subset:  IM    
Affiliation:
Department of Immunology, Peking University Health Science Center, Beijing 100083, China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AW921515
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
COS Cells
Cercopithecus aethiops
Chemokines, CXC / chemistry,  genetics*
Chemotaxis, Leukocyte
Chromosomes, Mammalian / genetics
Cloning, Molecular
DNA, Complementary / genetics
Humans
In Situ Hybridization
Lung / metabolism
Mice
Molecular Sequence Data
Physical Chromosome Mapping
RNA, Messenger / genetics,  metabolism
Rats
Sequence Alignment
Sequence Homology*
Chemical
Reg. No./Substance:
0/Chemokines, CXC; 0/Cxcl7 protein, rat; 0/DNA, Complementary; 0/RNA, Messenger

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