| Molecular cloning and characterization of a novel rat CXC chemokine, rPBP, the homologue of human and mouse PBP. | |
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MedLine Citation:
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PMID: 15016410 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously cloned the mouse platelet basic protein (mPBP), a homologue of human PBP, from mouse thymic stromal cells. Using EST alignment and RT-PCR, the rat homologue of human and mouse PBP was cloned from lung and named as rPBP. The complete open reading frame and part of the 3'- and 5'-non-coding regions were obtained through rapid amplification of cDNA ends. The rPBP cDNA encodes a protein of 111 amino acids containing a signal peptide of 37 amino acids at the N-terminus, with the mature protein of 74 amino acids. The rPBP is a new member of ELR+CXC chemokines. The mature protein of rPBP shares 69% and 45% homology with mouse and human PBP, respectively. In situ hybridization assay revealed rPBP to be predominantly localized in the pulmonary vascular endothelial cells. The eukaryotic expression vector pCDNA3-rPBP was constructed and transiently transfected into COS-7 cells. In the in vitro chemotaxis assay, the polymorphonuclear leukocytes (PMNs) were chemoattracted to the supernatants from transfected COS-7 cells in a dose-dependent manner. The implication of rPBP found in rat lung is that this chemokine may have the function to recruit PMNs to fight against pulmonary infection. |
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Authors:
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Wen-Xian Fu; Meng-Lei Zhu; Shun-You Gong; Yan Li; Wei-Feng Chen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cytokine Volume: 26 ISSN: 1043-4666 ISO Abbreviation: Cytokine Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-03-12 Completed Date: 2004-12-10 Revised Date: 2007-08-16 |
Medline Journal Info:
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Nlm Unique ID: 9005353 Medline TA: Cytokine Country: United States |
Other Details:
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Languages: eng Pagination: 37-43 Citation Subset: IM |
Affiliation:
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Department of Immunology, Peking University Health Science Center, Beijing 100083, China. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AW921515 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence COS Cells Cercopithecus aethiops Chemokines, CXC / chemistry, genetics* Chemotaxis, Leukocyte Chromosomes, Mammalian / genetics Cloning, Molecular DNA, Complementary / genetics Humans In Situ Hybridization Lung / metabolism Mice Molecular Sequence Data Physical Chromosome Mapping RNA, Messenger / genetics, metabolism Rats Sequence Alignment Sequence Homology* |
| Chemical | |
Reg. No./Substance:
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0/Chemokines, CXC; 0/Cxcl7 protein, rat; 0/DNA, Complementary; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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