| Molecular cloning and characterization of a novel mouse macrophage C-type lectin, mMGL2, which has a distinct carbohydrate specificity from mMGL1. | |
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MedLine Citation:
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PMID: 12016228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel mouse macrophage galactose-type C-type lectin 2 (mMGL2) was identified by BLAST analysis of expressed sequence tags. The sequence of mMGL2 is highly homologous to the mMGL, which should now be called mMGL1. The open reading frame of mMGL2 contains a sequence corresponding to a type II transmembrane protein with 332 amino acids having a single extracellular C-type lectin domain. The 3'-untranslated region included long terminal repeats of mouse early transposon. The Mgl2 gene was cloned from a 129/SvJ mouse genomic library and sequenced. The gene spans 7,136 base pairs and consists of 10 exons, which is similar to the genomic organization of mMGL1. The reverse transcriptase-PCR analysis indicates that mMGL2 is expressed in cell lines and normal mouse tissues in a macrophage-restricted manner, also very similar to that of mMGL1. The mMGL2 mRNA was also detected in mMGL1-positive cells, which were sorted from thioglycollate-induced peritoneal cells with a mMGL1-specific monoclonal antibody, LOM-8.7. The soluble recombinant proteins of mMGL2 exhibited carbohydrate specificity for alpha- and beta-GalNAc-conjugated soluble polyacrylamides, whereas mMGL1 preferentially bound Lewis X-conjugated soluble polyacrylamides in solid phase assays. These two lectins may function cooperatively as recognition and endocytic molecules on macrophages and related cells. |
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Authors:
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Makoto Tsuiji; Mayuko Fujimori; Yoshimi Ohashi; Nobuaki Higashi; Thandi M Onami; Stephen M Hedrick; Tatsuro Irimura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2002-05-16 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 277 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-05 Completed Date: 2002-09-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 28892-901 Citation Subset: IM |
Affiliation:
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Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3' Untranslated Regions Amino Acid Sequence Animals Antibodies, Monoclonal / metabolism Asialoglycoproteins Base Sequence Carbohydrate Metabolism Cell Line Cloning, Molecular Cytoplasm / metabolism DNA, Complementary / metabolism Dose-Response Relationship, Drug Endocytosis Exons Female Hydrogen-Ion Concentration Immunohistochemistry Lectins / biosynthesis*, genetics*, metabolism* Lectins, C-Type* Macrophages / metabolism* Membrane Proteins* Mice Mice, Inbred C57BL Molecular Sequence Data Phylogeny Protein Binding Protein Structure, Tertiary RNA, Messenger / metabolism Recombinant Fusion Proteins / metabolism Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/3' Untranslated Regions; 0/Antibodies, Monoclonal; 0/Asialoglycoproteins; 0/DNA, Complementary; 0/Lectins; 0/Lectins, C-Type; 0/MGL2 protein, mouse; 0/Membrane Proteins; 0/Mgl1 protein, mouse; 0/RNA, Messenger; 0/Recombinant Fusion Proteins; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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