Document Detail

Molecular cloning and characterization of the gene encoding mouse melanoma antigen by cDNA library transfection.
MedLine Citation:
PMID:  1380036     Owner:  NLM     Status:  MEDLINE    
We have isolated a cDNA (H52) of 2.8-kb-long encoding an 80-kDa mouse melanoma Ag that is defined by a syngeneic anti-B16 melanoma mAb with an ability to block anti-melanoma cytotoxic T cell responses. H52 transfectants were brightly stained with the antibody, and the 80-kDa molecule was immunoprecipitated from the transfectants. Northern blot analysis showed that this transcript was detected in mouse melanoma cells of C57BL/6 and DBA/2 origin, C1300 A/J neuroblastoma, L cell (C3H) and EL-4 T lymphoma (C57BL/6), faintly in BW5147 (AKR) T lymphoma, but not in other tumors, such as S913 fibrosarcoma (C57BL/10), NIH3T3, 70 Z/3 pre-B lymphoma, and P3U1 plasmacytoma (BALB/c). Since the transcripts were not found in normal C57BL/6 tissues of fetus, newborn, and adult origin, the H52 expression is associated with transforming phenotypes. However, no tissue- or cell type-specific expression was observed. Nucleotide sequence analysis has clearly demonstrated that H52 cDNA encodes the full length of the env gene and long terminal repeat region of endogenous ecotropic murine leukemia provirus of AKV-type, which is defective in C57BL/6. The H52 envelope protein has several amino acid changes compared to those of AKV, one of which is in the env 14 peptide region preferentially associated with MHC molecule, suggesting the possible reason for the difference of antibody reactivity even in H52-positive tumors. We also demonstrate that CTL against H52 transfectant kills B16 melanoma. Thus, the above results are direct evidence that even the endogenous self molecule, when constitutively expressed, does act as a tumor Ag.
H Hayashi; H Matsubara; T Yokota; I Kuwabara; M Kanno; H Koseki; K Isono; T Asano; M Taniguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  149     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-09-14     Completed Date:  1992-09-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1223-9     Citation Subset:  AIM; IM; X    
Division of Molecular Immunology, Chiba University, Japan.
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MeSH Terms
Amino Acid Sequence
Antigens, Neoplasm / genetics*,  immunology
Base Sequence
Cloning, Molecular
DNA / genetics
Gene Expression
Gene Products, env / genetics,  immunology*
Leukemia Virus, Murine / genetics*
Melanoma / genetics,  immunology*
Molecular Sequence Data
RNA, Messenger / genetics
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Epitopes; 0/Gene Products, env; 0/RNA, Messenger; 9007-49-2/DNA

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