Document Detail


Molecular cloning and characterization of crustacean type-one dopamine receptors: D1alphaPan and D1betaPan.
MedLine Citation:
PMID:  16426885     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dopamine (DA) differentially modulates identified neurons in the crustacean stomatogastric nervous system (STNS). While the electrophysiological actions of DA have been well characterized, little is known about the dopaminergic transduction cascades operating in this system. As a first step toward illuminating the molecular underpinnings of dopaminergic signal transduction in the crustacean STNS, we have cloned and characterized two type-one DA receptors (DARs) from the spiny lobster (Panulirus interruptus): D(1alphaPan) and D(1betaPan). We found that the structure and function of these arthropod DARs are well conserved across species. Using a heterologous expression system, we determined that DA, but not serotonin, octopamine, tyramine or histamine activates these receptors. When stably expressed in HEK cells, the D(1alphaPan) receptor couples with Gs, and DA elicits an increase in [cAMP]. The D(1betaPan) receptor responds to DA with a net increase in [cAMP] that is mediated by Gs and Gz.
Authors:
Merry C Clark; Deborah J Baro
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-01-19
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  143     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-28     Completed Date:  2006-05-17     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  294-301     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Cloning, Molecular
Conserved Sequence
Cyclic AMP / metabolism
Dopamine / pharmacology
GTP-Binding Protein alpha Subunits, Gs / metabolism
Humans
Molecular Sequence Data
Palinuridae / genetics,  metabolism,  physiology*
Receptors, Dopamine D1 / agonists,  genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
NS38770/NS/NINDS NIH HHS; R01 NS038770/NS/NINDS NIH HHS; R01 NS038770-05/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Dopamine D1; E0399OZS9N/Cyclic AMP; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gs; VTD58H1Z2X/Dopamine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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