| Molecular cloning and characterization of crustacean type-one dopamine receptors: D1alphaPan and D1betaPan. | |
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MedLine Citation:
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PMID: 16426885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dopamine (DA) differentially modulates identified neurons in the crustacean stomatogastric nervous system (STNS). While the electrophysiological actions of DA have been well characterized, little is known about the dopaminergic transduction cascades operating in this system. As a first step toward illuminating the molecular underpinnings of dopaminergic signal transduction in the crustacean STNS, we have cloned and characterized two type-one DA receptors (DARs) from the spiny lobster (Panulirus interruptus): D(1alphaPan) and D(1betaPan). We found that the structure and function of these arthropod DARs are well conserved across species. Using a heterologous expression system, we determined that DA, but not serotonin, octopamine, tyramine or histamine activates these receptors. When stably expressed in HEK cells, the D(1alphaPan) receptor couples with Gs, and DA elicits an increase in [cAMP]. The D(1betaPan) receptor responds to DA with a net increase in [cAMP] that is mediated by Gs and Gz. |
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Authors:
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Merry C Clark; Deborah J Baro |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-01-19 |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology Volume: 143 ISSN: 1096-4959 ISO Abbreviation: Comp. Biochem. Physiol. B, Biochem. Mol. Biol. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-02-28 Completed Date: 2006-05-17 Revised Date: 2010-09-29 |
Medline Journal Info:
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Nlm Unique ID: 9516061 Medline TA: Comp Biochem Physiol B Biochem Mol Biol Country: England |
Other Details:
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Languages: eng Pagination: 294-301 Citation Subset: IM |
Affiliation:
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Program for Cell and Molecular Biology and Physiology, Department of Biology, Georgia State University, P.O. Box 4010, Atlanta, 30303, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cloning, Molecular Conserved Sequence Cyclic AMP / metabolism Dopamine / pharmacology GTP-Binding Protein alpha Subunits, Gs / metabolism Humans Molecular Sequence Data Palinuridae / genetics, metabolism, physiology* Receptors, Dopamine D1 / agonists, genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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NS38770/NS/NINDS NIH HHS; R01 NS038770-05/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Dopamine D1; 60-92-4/Cyclic AMP; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gs |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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