Document Detail


Molecular cloning of PC3, a putatively secreted protein whose mRNA is induced by nerve growth factor and depolarization.
MedLine Citation:
PMID:  1849653     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PC3 is an immediate early gene induced by nerve growth factor in PC12 cells, a cell line derived from a tumor of the adrenal medulla that undergoes neuronal differentiation in the presence of nerve growth factor. This induction is independent of new protein synthesis as it can occur in the presence of cycloheximide. PC3 is also induced with similar kinetics, but at lower levels, by membrane depolarization (both in vivo and in vitro) and epidermal growth factor. It is induced at much lower levels by fibroblast growth factor and interleukin 6. In vivo it is found expressed in tissues, such as brain at embryonic day 13.5, placenta, amnion, and spleen, which are proliferating and/or differentiating. The deduced protein sequence from the cDNA indicates the presence of a signal peptide, suggesting that PC3 is secreted.
Authors:
A Bradbury; R Possenti; E M Shooter; F Tirone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  88     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-05-15     Completed Date:  1991-05-15     Revised Date:  2010-02-05    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3353-7     Citation Subset:  IM    
Affiliation:
Istituto di Neurobiologia, Consiglio Nazionale delle Ricerche, Rome, Italy.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M60361;  M60362;  M60363;  M60364;  M60365;  M60366;  M60367;  M60368;  M60369;  M60921
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MeSH Terms
Descriptor/Qualifier:
Adrenal Gland Neoplasms / genetics*
Amino Acid Sequence
Animals
Base Sequence
Bucladesine / pharmacology
Cloning, Molecular
DNA / genetics
Epidermal Growth Factor / pharmacology
Fibroblast Growth Factors / pharmacology
Gene Expression / drug effects
Immediate-Early Proteins / genetics*,  secretion
Interleukin-6 / pharmacology
Membrane Potentials / drug effects*
Molecular Sequence Data
Neoplasm Proteins / genetics*,  secretion
Nerve Growth Factors / pharmacology*
Nerve Tissue Proteins / genetics*,  secretion
Pentylenetetrazole / pharmacology
Pheochromocytoma / genetics*
Potassium Chloride / pharmacology
RNA, Messenger / genetics
Rats
Restriction Mapping
Tetradecanoylphorbol Acetate / pharmacology
Tissue Distribution
Grant Support
ID/Acronym/Agency:
NS 04270/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/BTG2 protein, rat; 0/Immediate-Early Proteins; 0/Interleukin-6; 0/Neoplasm Proteins; 0/Nerve Growth Factors; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 16561-29-8/Tetradecanoylphorbol Acetate; 362-74-3/Bucladesine; 54-95-5/Pentylenetetrazole; 62031-54-3/Fibroblast Growth Factors; 62229-50-9/Epidermal Growth Factor; 7447-40-7/Potassium Chloride; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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