| Molecular cloning of the Corynebacterium glutamicum ('Brevibacterium lactofermentum' AJ12036) odhA gene encoding a novel type of 2-oxoglutarate dehydrogenase. | |
| | |
MedLine Citation:
|
PMID: 9004499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The Corynebacterium glutamicum ('Brevibacterium lactofermentum' AJ12036) odhA gene, encoding 2-oxoglutarate dehydrogenase (E1o subunit of the 2-oxoglutarate dehydrogenase complex), has been isolated and identified as an homologous counterpart of the Escherichia coll sucA and Bacillus subtilis odhA genes. The nucleotide sequence of a 4394 bp chromosomal fragment containing the C. glutamicum odhA gene was determined. The odhA gene comprised 3771 bp (1257 codons, including the initiation codon) and a molecular mass of 138656 Da was predicted for the OdhA polypeptide. Northern blot analysis revealed a 3.9 kb transcript. The size of the transcript, together with the presence of a rho-independent terminator-like structure, suggests that C. glutamicum odhA is monocistronic. Cells harbouring plasmids carrying C. glutamicum odhA showed a threefold increase in specific 2-oxoglutarate dehydrogenase complex activity and expression of a protein with an apparent molecular mass of 136 kDa, in good agreement with the predicted size of the OdhA polypeptide. The C-terminal region of the C. glutamicum OdhA protein shows strong sequence similarity to E1os from other organisms. C. glutamicum OdhA has an N-terminal extension not found in previously reported E1os. The amino acid sequence of this extension shows similarity to that of the C-terminal region of dihydrolipoamide S-succinyltransferase (E2o) subunits of 2-oxoglutarate dehydrogenase complexes and dihydrolipoamide S-acetyltransferase (E2p) subunits of pyruvate dehydrogenase complexes. It suggests that the C. glutamicum odhA gene might encode a novel bifunctional protein with E1o and E2o activities. |
| | |
Authors:
|
Y Usuda; N Tujimoto; C Abe; Y Asakura; E Kimura; Y Kawahara; O Kurahashi; H Matsui |
Related Documents
:
|
7630939 - C4 isoform of nadp-malate dehydrogenase. cdna cloning and expression in leaves of c4, c... 3515319 - Isolation of human glucose-6-phosphate dehydrogenase (g6pd) cdna clones: primary struct... 12867459 - Is981-mediated adaptive evolution recovers lactate production by ldhb transcription act... 17668199 - Biochemical characterization and phylogenetic analysis of udp-glucose dehydrogenase fro... 2253629 - Cloning and sequence analysis of the genes encoding the dihydrolipoamide acetyltransfer... 3754749 - A postmeiotically expressed clone encodes lactate dehydrogenase isozyme x. 11062009 - Caspase-14: analysis of gene structure and mrna expression during keratinocyte differen... 8336129 - Nucleotide sequence of beet cryptic virus 3 dsrna2 which encodes a putative rna-depende... 17657539 - A novel phytase appa from citrobacter amalonaticus cgmcc 1696: gene cloning and overexp... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Microbiology (Reading, England) Volume: 142 ( Pt 12) ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 1996 Dec |
Date Detail:
|
Created Date: 1997-02-27 Completed Date: 1997-02-27 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9430468 Medline TA: Microbiology Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 3347-54 Citation Subset: IM |
Affiliation:
|
Central Research Laboratories, AJINOMOTO Co., Inc., Kawasaki-shi, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/D84102 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Bacillus subtilis / enzymology Base Sequence Chromosomes, Bacterial Cloning, Molecular Codon Corynebacterium / enzymology*, genetics* Genes, Bacterial* Ketoglutarate Dehydrogenase Complex / biosynthesis*, chemistry, genetics Macromolecular Substances Molecular Sequence Data Pyruvate Dehydrogenase Complex / chemistry Recombinant Proteins / biosynthesis, chemistry Restriction Mapping Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
|
0/Codon; 0/Macromolecular Substances; 0/Pyruvate Dehydrogenase Complex; 0/Recombinant Proteins; EC 1.2.4.2/Ketoglutarate Dehydrogenase Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Metabolic adaptation of Trichomonas vaginalis to growth rate and glucose availability.
Next Document: Insertional gene inactivation in a phototrophic sulphur bacterium: APS-reductase-deficient mutants o...