Document Detail

Molecular cloning of a C-type lectin (LvLT) from the shrimp Litopenaeus vannamei: early gene down-regulation after WSSV infection.
MedLine Citation:
PMID:  17276083     Owner:  NLM     Status:  MEDLINE    
C-type lectin is one of the pattern-recognition proteins of the non-self innate immune system in the invertebrates. In this study, a lectin-like cDNA (LvLT) of Litopenaeus vannamei was cloned and characterized. LvLT cDNA consists of 1035 nt encoding for a protein with 345 amino acid residues. The deduced LvLT consists of two putative carbohydrate-recognition domains (CRDs) as found in most C-type lectins. The first CRD consists of an amino acid motif (QPD) for the binding of galactose and the other CRDs consist of amino acid motifs (EPN) for the binding of mannose. Except for some conserved amino acid residues, the CRD of LvLT shared an overall low amino acid sequence identity with CRDs of other lectins. Unlike other shrimp lectins, LvLT is expressed only in the hepatopancreas but not in the hemocytes as revealed by RT-PCR. When juvenile shrimp were challenged with shrimp extracts containing white spot syndrome virus (WSSV), the expression levels of LvLT decreased initially in the first 2 h and then increased to a much higher level after 4 h. The results suggest that the initial reduction in LvLT transcript level may be related to the WSSV infection in shrimp.
Tracy Hoi Tung Ma; Shirley Hiu Kwan Tiu; Jian-Guo He; Siu-Ming Chan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-19
Journal Detail:
Title:  Fish & shellfish immunology     Volume:  23     ISSN:  1050-4648     ISO Abbreviation:  Fish Shellfish Immunol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-05-15     Completed Date:  2007-10-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9505220     Medline TA:  Fish Shellfish Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  430-7     Citation Subset:  IM    
Department of Zoology, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA Virus Infections / genetics,  immunology,  veterinary*,  virology
DNA, Viral / genetics
Lectins, C-Type / biosynthesis,  genetics*,  immunology
Molecular Sequence Data
Penaeidae / genetics*,  immunology,  virology
RNA, Viral / genetics
Random Amplified Polymorphic DNA Technique / veterinary
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Sequence Alignment
White spot syndrome virus 1 / immunology*
Reg. No./Substance:
0/DNA, Viral; 0/Lectins, C-Type; 0/RNA, Viral

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