| Molecular cloning of a C-type lectin (LvLT) from the shrimp Litopenaeus vannamei: early gene down-regulation after WSSV infection. | |
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MedLine Citation:
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PMID: 17276083 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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C-type lectin is one of the pattern-recognition proteins of the non-self innate immune system in the invertebrates. In this study, a lectin-like cDNA (LvLT) of Litopenaeus vannamei was cloned and characterized. LvLT cDNA consists of 1035 nt encoding for a protein with 345 amino acid residues. The deduced LvLT consists of two putative carbohydrate-recognition domains (CRDs) as found in most C-type lectins. The first CRD consists of an amino acid motif (QPD) for the binding of galactose and the other CRDs consist of amino acid motifs (EPN) for the binding of mannose. Except for some conserved amino acid residues, the CRD of LvLT shared an overall low amino acid sequence identity with CRDs of other lectins. Unlike other shrimp lectins, LvLT is expressed only in the hepatopancreas but not in the hemocytes as revealed by RT-PCR. When juvenile shrimp were challenged with shrimp extracts containing white spot syndrome virus (WSSV), the expression levels of LvLT decreased initially in the first 2 h and then increased to a much higher level after 4 h. The results suggest that the initial reduction in LvLT transcript level may be related to the WSSV infection in shrimp. |
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Authors:
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Tracy Hoi Tung Ma; Shirley Hiu Kwan Tiu; Jian-Guo He; Siu-Ming Chan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-19 |
Journal Detail:
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Title: Fish & shellfish immunology Volume: 23 ISSN: 1050-4648 ISO Abbreviation: Fish Shellfish Immunol. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-05-15 Completed Date: 2007-10-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9505220 Medline TA: Fish Shellfish Immunol Country: England |
Other Details:
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Languages: eng Pagination: 430-7 Citation Subset: IM |
Affiliation:
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Department of Zoology, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/DQ871245 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Virus Infections / genetics, immunology, veterinary*, virology DNA, Viral / genetics Down-Regulation Lectins, C-Type / biosynthesis, genetics*, immunology Molecular Sequence Data Penaeidae / genetics*, immunology, virology RNA, Viral / genetics Random Amplified Polymorphic DNA Technique / veterinary Reverse Transcriptase Polymerase Chain Reaction / veterinary Sequence Alignment White spot syndrome virus 1 / immunology* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Lectins, C-Type; 0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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