Document Detail

Molecular cloning of a 2',3'-cyclic nucleotide 3'-phosphodiesterase: mRNAs with different 5' ends encode the same set of proteins in nervous and lymphoid tissues.
MedLine Citation:
PMID:  3040924     Owner:  NLM     Status:  MEDLINE    
Antibodies raised to a mixture of the 46 and 48 kDa rat CNS 2',3'-cyclic nucleotide 3-phosphodiesterases (CNPs) recognized apparently identical proteins in peripheral nervous system (PNS), thymus, and circulating blood lymphocytes. These antibodies were used to identify, in a rat brain phage lambda gt11 expression library, cDNA clones encoding beta-galactosidase-CNP fusion proteins, some of which showed CNP activity. In RNA blots, the subcloned CNP cDNA inserts hybridized to mRNAs of approximately 2400 and approximately 2800 nucleotides (nts), and to a approximately 2500 nt mRNA from thymus. Several nonexpressing CNP cDNAs were identified by plaque hybridization, and the mRNA transcribed in vitro from one of these cDNAs (pCNP7) encoded a complete 46 kDa CNP polypeptide. Examination of the deduced amino acid sequence revealed an apparent homology to cAMP binding sites in several other proteins. A 373 bp segment from the 5' end of this pCNP7 hybridized only to the 2800 nt nervous system mRNAs, thus revealing that not all CNP mRNAs share the same 5'-ends. Genomic DNA blots probed with CNP cDNAs suggest that there is a single gene which can be alternatively spliced to produce the various mRNA transcripts in the nervous and lymphoid tissues.
L Bernier; F Alvarez; E M Norgard; D W Raible; A Mentaberry; J G Schembri; D D Sabatini; D R Colman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  7     ISSN:  0270-6474     ISO Abbreviation:  J. Neurosci.     Publication Date:  1987 Sep 
Date Detail:
Created Date:  1987-10-21     Completed Date:  1987-10-21     Revised Date:  2012-04-16    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2703-10     Citation Subset:  IM    
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MeSH Terms
2',3'-Cyclic-Nucleotide Phosphodiesterases / genetics*
Amino Acid Sequence
Bacteriophage lambda / genetics
Base Sequence
Cloning, Molecular*
DNA / metabolism
Gene Expression Regulation
Lymphocytes / enzymology*
Molecular Weight
Peripheral Nerves / enzymology*
Phosphoric Diester Hydrolases*
RNA, Messenger / metabolism*
Recombinant Fusion Proteins / metabolism
Thymus Gland / enzymology*
Grant Support
Reg. No./Substance:
0/RNA, Messenger; 0/Recombinant Fusion Proteins; 9007-49-2/DNA; EC 3.1.4.-/2',3'-Cyclic-Nucleotide Phosphodiesterases; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC',3'-cyclic nucleotide 3'-phosphodiesterase

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