Document Detail


Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom.
MedLine Citation:
PMID:  15683874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A fibrinogen-clotting enzyme designed as jerdonobin-II was isolated from the venom of Trimeresurus jerdonii. It differed in molecular weight and N-terminal sequence with the previously isolated jerdonobin, a thrombin-like enzyme from the same venom. The enzyme consists of a single polypeptide chain with molecular weights of 30,000 and 32,000 under non-reducing and reducing conditions, respectively. Jerdonobin-II showed weak fibrinogen clotting activity and its activity unit on fibrinogen was calculated to be less than one unit using human thrombin as standard. The precursor protein sequence of jerodonobin-II was deduced from cloned cDNA sequence. The sequence shows high similarity (identity=89%) to TSV-PA, a specific plasminogen activator from venom of T. stejnegeri. Despite of the sequence similarity, jerdonobin-II was found devoid of plasminogen activating effect. Sequence alignment analysis suggested that the replacement of Lys239 in TSV-PA to Gln239 in jerdonobin-II might play an important role on their plasminogen activating activity difference.
Authors:
Yang Jin; Qiu-Min Lu; Run-Qiang Chen; Jian-Bo Wu; Yu-Liang Xiong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-12-20
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  45     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-01     Completed Date:  2005-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  353-60     Citation Subset:  IM    
Affiliation:
Department of Animal Toxinology, Kunming Institute of Zoology, the Chinese Academy of Sciences, East Jiao-Chang Road, Kunming 650223, Yunnan, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blood Coagulation / drug effects*
Crotalid Venoms / enzymology*
Fibrinogen / chemistry*
Molecular Sequence Data
Sequence Alignment
Sequence Homology, Amino Acid
Serine Endopeptidases / chemistry*,  isolation & purification,  metabolism
Substrate Specificity
Trimeresurus
Chemical
Reg. No./Substance:
0/Crotalid Venoms; 9001-32-5/Fibrinogen; EC 3.4.21.-/Serine Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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