| Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom. | |
| | |
MedLine Citation:
|
PMID: 15683874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A fibrinogen-clotting enzyme designed as jerdonobin-II was isolated from the venom of Trimeresurus jerdonii. It differed in molecular weight and N-terminal sequence with the previously isolated jerdonobin, a thrombin-like enzyme from the same venom. The enzyme consists of a single polypeptide chain with molecular weights of 30,000 and 32,000 under non-reducing and reducing conditions, respectively. Jerdonobin-II showed weak fibrinogen clotting activity and its activity unit on fibrinogen was calculated to be less than one unit using human thrombin as standard. The precursor protein sequence of jerodonobin-II was deduced from cloned cDNA sequence. The sequence shows high similarity (identity=89%) to TSV-PA, a specific plasminogen activator from venom of T. stejnegeri. Despite of the sequence similarity, jerdonobin-II was found devoid of plasminogen activating effect. Sequence alignment analysis suggested that the replacement of Lys239 in TSV-PA to Gln239 in jerdonobin-II might play an important role on their plasminogen activating activity difference. |
| | |
Authors:
|
Yang Jin; Qiu-Min Lu; Run-Qiang Chen; Jian-Bo Wu; Yu-Liang Xiong |
Related Documents
:
|
19470354 - Transcriptome analysis of the amazonian viper bothrops atrox venom gland using expresse... 3513184 - Expression of a cdna derived from the yeast killer preprotoxin gene: implications for p... 8740424 - Molecular cloning of a third chitinase gene (cht1) from candida albicans. 10627034 - Family 19 chitinases of streptomyces species: characterization and distribution. 1656734 - Recombinational biases in the rearranged c1-inhibitor genes of hereditary angioedema pa... 14870774 - The molecular basis of thyroid epithelial tumorigenesis. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-12-20 |
Journal Detail:
|
Title: Toxicon : official journal of the International Society on Toxinology Volume: 45 ISSN: 0041-0101 ISO Abbreviation: Toxicon Publication Date: 2005 Mar |
Date Detail:
|
Created Date: 2005-02-01 Completed Date: 2005-05-03 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 1307333 Medline TA: Toxicon Country: England |
Other Details:
|
Languages: eng Pagination: 353-60 Citation Subset: IM |
Affiliation:
|
Department of Animal Toxinology, Kunming Institute of Zoology, the Chinese Academy of Sciences, East Jiao-Chang Road, Kunming 650223, Yunnan, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Base Sequence Blood Coagulation / drug effects* Crotalid Venoms / enzymology* Fibrinogen / chemistry* Molecular Sequence Data Sequence Alignment Sequence Homology, Amino Acid Serine Endopeptidases / chemistry*, isolation & purification, metabolism Substrate Specificity Trimeresurus |
| Chemical | |
Reg. No./Substance:
|
0/Crotalid Venoms; 9001-32-5/Fibrinogen; EC 3.4.21.-/Serine Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Acute intoxication by Crotalaria retusa in sheep.
Next Document: Involvement of phospholipase C in Yersinia enterocolitica heat stable enterotoxin (Y-STa) mediated r...