Document Detail


Molecular characterization, phylogeny, and expression of c-type and g-type lysozymes in brill (Scophthalmus rhombus).
MedLine Citation:
PMID:  18479938     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lysozymes are key proteins of the innate immune system against bacterial infections. In this study we report the molecular cloning and characterization of the c-type and g-type lysozymes in brill (Scophthalmus rhombus). Catalytic and other conserved residues required for functionality were identified. Phylogenetic analysis revealed distinct evolutionary histories for each lysozyme type. Expression profiles of both lysozyme genes were studied in juvenile tissues using a real-time PCR approach. c-Type lysozyme was expressed mainly in stomach and liver, whereas the g-type was detected in all tissues with highest mRNA levels observed in the spleen. Induction experiments revealed that g-type transcripts increased significantly in head kidney after lipopolysaccharide (25- and 23-fold at 12 and 24h, respectively) and Photobacterium damselae subsp. piscicida (17-fold at 24h) treatments. In contrast, no induction was observed for c-type lysozyme. All these data suggest that g-type lysozyme is involved in the response against bacterial infections, whereas c-type lysozyme may also play a role in digestion.
Authors:
Rosa M Jiménez-Cantizano; Carlos Infante; Beatriz Martin-Antonio; Marian Ponce; Ismael Hachero; Jose Ignacio Navas; Manuel Manchado
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-24
Journal Detail:
Title:  Fish & shellfish immunology     Volume:  25     ISSN:  1050-4648     ISO Abbreviation:  Fish Shellfish Immunol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-13     Completed Date:  2008-09-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9505220     Medline TA:  Fish Shellfish Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  57-65     Citation Subset:  IM    
Affiliation:
IFAPA Centro El Toruño, Consejería de Innovación Ciencia y Empresa, Junta de Andalucía, Molecular Biology Laboratory, Camino Tiro de pichón s/n, 11500 El Puerto de Santa María, Cádiz, Spain.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB355630;  AB355631
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Fish Diseases / enzymology,  microbiology
Flatfishes / genetics*,  metabolism*
Gene Expression Regulation, Enzymologic* / drug effects
Gram-Negative Bacterial Infections / enzymology,  veterinary
Lipopolysaccharides / pharmacology
Molecular Sequence Data
Muramidase / classification*,  genetics*
Photobacterium / immunology
Phylogeny*
Sequence Alignment
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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