Document Detail


Molecular characterization of a novel isolate of Candida tropicalis for enhanced lipid production.
MedLine Citation:
PMID:  23311514     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIM: To characterize a new isolate of Candida tropicalis for its enhanced storage lipid accumulation with respect to lipid composition, fatty acid profile and transcriptional regulation of four key genes involved in lipid productivity using different carbon sources. METHODS AND RESULTS: Upon growing C. tropicalis on various carbon substrates, glucose was found to be the best followed by xylose for the production of both biomass and storage lipid. On glucose (100 g l(-1) ) medium having specific nitrogen-stress (C:N 150:1), the yeast was capable of yielding about 58% lipid content of its dry biomass, and neutral lipid accounted for about 75% of the total lipid. Fatty acid profiles revealed that the glucose contributed to the highest yield of total fatty acids, maximum proportion of saturated fatty acids and a significant amount of oleic acid. The enhanced lipid production with specific fatty acid profile correlated to the strong up-regulation of acetyl Co-A carboxylase, stearoyl-ACP desaturase and diacylglycerol acyltransferase genes but not the malic enzyme gene. CONCLUSIONS: Together, the results documented the differential regulation of four genes of lipid biosynthesis in the newly isolated C. tropicalis oleaginous strain by various carbon sources. © 2013 No claim to US Government works.
Authors:
Prabuddha Dey; Mrinal K Maiti
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-14
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 No claim to US Government works.
Affiliation:
Advanced Laboratory for Plant Genetic Engineering, Advanced Technology Development Centre, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
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