Document Detail

Molecular characterization of neoplastic and normal 'sister' lymphoblastoid B-cell lines from chronic lymphocytic leukemia.
MedLine Citation:
PMID:  23297799     Owner:  NLM     Status:  Publisher    
Abstract Chronic lymphocytic leukemia (CLL) B-cells resemble self-renewing CD5(+) B-cells carrying auto/xeno-antigen-reactive B-cell receptors (BCRs), and multiple innate pattern-recognition receptors, such as Toll-like receptors and scavenger-receptors. Integration of signals from BCRs with multiple surface membrane-receptors determines whether the cells will be proliferating, anergic, or apoptotic. To better understand to role of antigen in leukemogenesis, CLL cell lines producing monoclonal antibodies (mAbs) will facilitate structural analysis of antigens and supply DNA for genetic studies. We present here a comprehensive genotypic and phenotypic characterization of available CLL and normal B-cell-derived lymphoblastoid cell lines (LCLs) from the same individuals (n=17). Authenticity and verification studies of CLL-patient origin were done by IGHV-sequencing, FISH, DNA/STR-fingerprinting. Innate B-cell features, ie. natural Ab-production and CD5-receptors, were present in most CLL cell lines, but in none of the normal LCLs. This panel of immortalized CLL-derived cell lines is a valuable reference representing a renewable source of authentic Abs and DNA.
Anna Lanemo Myhrinder; Eva Hellqvist; Ann-Charlotte Bergh; Mattias Jansson; Kenneth Nilsson; Per Hultman; Jon Jonasson; Anne Mette Buhl; Lone Bredo Pedersen; Jesper Jurlander; Eva Klein; Nicole Weit; Marco Herling; Richard Rosenquist; Anders Rosén
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  Leukemia & lymphoma     Volume:  -     ISSN:  1029-2403     ISO Abbreviation:  Leuk. Lymphoma     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9007422     Medline TA:  Leuk Lymphoma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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