| Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and their relationship to human isolates. | |
MedLine Citation:
|
PMID: 16387748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: Methicillin-resistant Staphylococcus aureus (MRSA) isolates from pet animals were characterized and compared with human isolates from clonal complexes most prevalent in Central Europe. METHODS: S. aureus isolates were investigated for their in vitro susceptibility to antimicrobial agents by broth microdilution. Resistance genes and the Panton-Valentine leucocidin gene lukF-lukS were identified by PCR. All isolates were characterized by SmaI macrorestriction analysis and spa typing to assess their genomic relationships. Representative isolates were additionally analysed by multilocus sequence typing and PCR-directed SCCmec typing. RESULTS: All pet isolates carried the resistance genes mecA and erm(C) and proved to be resistant to beta-lactams and MLS(B) antibiotics. In addition, all isolates were resistant to fluoroquinolones. None of the pet isolates carried the Panton-Valentine leucocidin gene lukF-lukS. Macrorestriction analysis revealed that the pet MRSA isolates exhibited four closely related SmaI fragment patterns. Moreover, all isolates revealed spa type t032. Further analysis of representatives of the different PFGE types revealed the presence of multilocus sequence type ST22 in combination with a type IV SCCmec element. Thus, molecular typing results were similar for pet strains and human ST22 reference strains. CONCLUSIONS: Based on their strain characteristics, the MRSA isolates from pets investigated in this study closely resembled ST22 MRSA isolates which are widely disseminated in German hospitals. The results of this study indicate that cross-transmission of MRSA between pet animals and humans might have occurred. |
Authors:
|
Birgit Strommenger; Corinna Kehrenberg; Christiane Kettlitz; Christa Cuny; Jutta Verspohl; Wolfgang Witte; Stefan Schwarz |
Related Documents
:
|
11368798 - Percutaneous treatment of chronic mrsa osteomyelitis with a novel plant-derived antisep... 17427698 - A comparison of three bioassay techniques to determine amitraz susceptibility in boophi... 8408938 - Resistance to methicillin and virulence of staphylococcus aureus strains in bacteriemic... 12837728 - Evaluation of a cefoxitin 30 microg disc on iso-sensitest agar for detection of methici... 8494378 - Ecology of haemophilus influenzae and haemophilus parainfluenzae in sputum and saliva a... 3009408 - Nodulation inhibition by rhizobium leguminosarum multicopy nodabc genes and analysis of... |
Publication Detail:
|
Type: Journal Article Date: 2005-12-30 |
Journal Detail:
|
Title: The Journal of antimicrobial chemotherapy Volume: 57 ISSN: 0305-7453 ISO Abbreviation: J. Antimicrob. Chemother. Publication Date: 2006 Mar |
Date Detail:
|
Created Date: 2006-02-07 Completed Date: 2006-05-10 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7513617 Medline TA: J Antimicrob Chemother Country: England |
Other Details:
|
Languages: eng Pagination: 461-5 Citation Subset: IM |
Affiliation:
|
Robert Koch Institute, Wernigerode Branch, D-38855 Wernigerode, Germany. strommengerb@rki.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cat Diseases / microbiology* Cats Dog Diseases / microbiology* Dogs Genotype Humans Methicillin Resistance* Molecular Epidemiology Phenotype Phylogeny Staphylococcal Infections / microbiology*, veterinary* Staphylococcus aureus / drug effects*, genetics |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Safety and factors predicting the duration of first and second treatment interruptions guided by CD4...
Next Document: In vitro pharmacodynamics of rapid versus continuous infusion of amphotericin B deoxycholate against...