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Molecular characterization of lactic acid bacteria and in situ amylase expression during traditional fermentation of cereal foods.
MedLine Citation:
PMID:  22608231     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Lactic acid bacteria play an important role in traditional fermented foods consumed in different countries. Study of their taxonomic structure and diversity is necessary for starter culture selection, improved safety and nutritional enhancement. To achieve these objectives, microbial genomic typing methods were used to study genetic differences of autochthonous bacteria and their distribution in two traditional African fermented cereal foods. A total of 85 predominant bacterial species were isolated from ogi and kunu-zaki obtained from Northern and Southern geographical region of Nigeria. They were identified using combination of 16S rRNA gene sequencing, multilocus sequence analysis (MLSA) based on rpoA, pheS and atpA genes as well as M13-PCR gel fingerprints. The results showed that Lactobacillus fermentum was the most frequently isolated species in ogi (71.4%) and kunu-zaki (84.5%). Other species of lactic acid bacteria (LAB) identified were Lactobacillus plantarum, Streptococcus gallolyticus subsp. macedonicus and Pediococcus pentosaceus. Non lactic acid bacteria isolated from these foods were species belonging to the Bacillus and Staphylococcus. Non-metric multidimensional scaling (nMDS) analysis of the M13-PCR fingerprints for LAB strains showed clonal diversity among strains of the same species. In vitro and in situ expression of amylase gene during fermentation by amylolytic L. plantarum ULAG11 was detected, indicating the potential usefulness of such species for development of starter cultures and for controlled fermentation processes.
Authors:
Folarin Anthony Oguntoyinbo; Arjan Narbad
Publication Detail:
Type:  Journal Article     Date:  2012-03-21
Journal Detail:
Title:  Food microbiology     Volume:  31     ISSN:  1095-9998     ISO Abbreviation:  Food Microbiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-05-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8601127     Medline TA:  Food Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  254-62     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Institute of Food Research, Norwich Research Park, Conley Lane, Norwich, United Kingdom; Department of Microbiology, Faculty of Science, University of Lagos, Akoka, Lagos, Nigeria.
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