Document Detail

Molecular characterization of invasive subpopulations from an esophageal squamous cell carcinoma cell line.
MedLine Citation:
PMID:  20392990     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Once diagnosed, esophageal cancer has a very low overall 5-year survival rate. This study investigates the mechanisms behind the invasiveness and severity of esophageal squamous cell carcinoma (ESCC). MATERIALS AND METHODS: Transwell invasion chamber was used to subdivide one Taiwanese ESCC cell line, CE81T/VGH, into sublines (CE81T-0, CE81T-1, CE81T-2, CE81T-3, and CE81T-4) in four rounds of assays; the most invasive were identified, and various factors related to their invasiveness measured. RESULTS: CE81T-1, CE81T-2, CE81T-3 and CE81T-4 sublines were significantly more invasive than the parental cells (CE81T/VGH) and CE81T-0 subline. CE81T-1 and CE81T-4, the sublines we chose to study further, had significantly greater colony-forming ability (3.5- to 2.7-fold) and wound migrating activity (1.95- to 2.6-fold) than the parental cells in vitro (p<0.01). They also displayed greater tumorigenesis in immunodeficient BALB/c Foxlnn mice than the parental cells. We found an inverse correlation between expression of tissue inhibitor of metalloproteinase-2 and invasive ability, and a significant positive correlation between expressions of matrix metalloproteinase-1, vimentin, and p-Src (pY416) in these cell lines and their invasiveness (all p<0.05). CONCLUSION: The subline model may be used to study the molecular and genetic mechanisms underlying the invasion and metastasis of ESCC.
Yu-Kuei Chen; Wun-Shaing Wayne Chang; I-Chen Wu; Ling-Hui Li; Sheau-Fang Yang; Jeff Yi-Fu Chen; Ming-Chung Hsu; Shih-Hsien Chen; Deng-Chyang Wu; Jang-Ming Lee; Chun-Hsiung Huang; Yih-Gang Goan; Shah-Hwa Chou; Chia-Tsuan Huang; Ming-Tsang Wu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-15     Completed Date:  2010-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  727-36     Citation Subset:  IM    
Department of Family Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, ROC.
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MeSH Terms
Blotting, Western
Carcinoma, Squamous Cell / genetics,  metabolism,  pathology*
Cell Growth Processes / physiology
Cell Line, Tumor
Cell Movement / physiology
Esophageal Neoplasms / genetics,  metabolism,  pathology*
Flow Cytometry
Matrix Metalloproteinase 1 / biosynthesis,  genetics
Matrix Metalloproteinase 2 / biosynthesis,  genetics
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Neoplasm Invasiveness
Neoplasm Metastasis
RNA, Messenger / biosynthesis,  genetics
Reverse Transcriptase Polymerase Chain Reaction
Tissue Inhibitor of Metalloproteinase-2 / biosynthesis
Vimentin / biosynthesis,  genetics
Reg. No./Substance:
0/RNA, Messenger; 0/Vimentin; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; EC Metalloproteinase 2; EC Metalloproteinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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