Document Detail


Molecular characterization of an inhibitor of apoptosis in the Egyptian armyworm, Spodoptera littoralis, and midgut cell death during metamorphosis.
MedLine Citation:
PMID:  17967343     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cDNA corresponding to an inhibitor of apoptosis (IAP) from the Egyptian armyworm, Spodoptera littoralis, was cloned by RT-PCR. Sequence analysis showed that the IAP of S. littoralis (SlIAP) contains two baculoviral IAP repeat (BIR) motifs, followed by a RING finger, an organization which is very similar to that of other lepidopteran IAPs. SlIAP mRNA was detected in ovary, testis, salivary gland, fat body, epidermis, brain and midgut of S. littoralis. During the last larval instar, prepupal and pupal stages, brain mRNA levels remained approximately constant, whereas those of midgut showed a large peak centred in the prepupal stage. Midgut morphology changed during metamorphosis from a semi-transparent, cylindrical structure in last instar larvae to a brownish globular mass in pupae. TUNEL assays, LysoTracker staining and caspase-3 immunohistochemistry, indicated that programmed cell death in midgut starts actively at the onset of pupation process, coinciding with the dramatic decrease of SlIAP mRNA levels observed at the same time.
Authors:
Lluïsa Vilaplana; Nuria Pascual; Nathalia Perera; Xavier Bellés
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-06
Journal Detail:
Title:  Insect biochemistry and molecular biology     Volume:  37     ISSN:  0965-1748     ISO Abbreviation:  Insect Biochem. Mol. Biol.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-10-30     Completed Date:  2008-02-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207282     Medline TA:  Insect Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1241-8     Citation Subset:  IM    
Affiliation:
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona (CSIC), Jordi Girona, 18-26, 08034 Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Amines / diagnostic use
Amino Acid Sequence
Animals
Brain / metabolism
Caspase 3 / metabolism
Cell Death / physiology*
Gastrointestinal Tract / growth & development,  metabolism
Gene Expression
In Situ Nick-End Labeling
Inhibitor of Apoptosis Proteins / chemistry,  genetics,  metabolism*
Metamorphosis, Biological / physiology*
Molecular Sequence Data
Molecular Structure
Sequence Analysis, DNA
Spodoptera / genetics,  growth & development,  metabolism*
Chemical
Reg. No./Substance:
0/Amines; 0/Inhibitor of Apoptosis Proteins; 0/Red DND-99; EC 3.4.22.-/Caspase 3

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