Document Detail

Molecular characterization of helodermin-preferring VIP receptors in SUP T1 lymphoma cells: evidence for receptor glycosylation.
MedLine Citation:
PMID:  1656036     Owner:  NLM     Status:  MEDLINE    
Cross-linking of [125I]helodermin to human SUP-T1 lymphoblasts with bis[2-(succinimidooxycarbonyloxy)ethyl]sulfone (BSOCOES) revealed a 63 K binding protein. This cross-linking was inhibited by helodermin and VIP. In cells submitted for 3-4 days to 0.2 microgram/ml tunicamycin, the Mr of an increasing proportion of helodermin-preferring receptors was reduced to 50 K and the total number of receptors was decreased by about 50%, without alteration in binding affinity and specificity. In parallel, the VIP-mediated adenylate cyclase stimulation was reduced by 30% with no change in NaF-, Gpp[NH]p-, and PGE1-stimulations. We conclude that a proper N-glycosylation of helodermin-preferring VIP receptors is required for normal receptor targeting and turnover but not for ligand binding and adenylate cyclase coupling.
P Gourlet; P Robberecht; J Christophe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of receptor research     Volume:  11     ISSN:  0197-5110     ISO Abbreviation:  J. Recept. Res.     Publication Date:  1991  
Date Detail:
Created Date:  1991-11-12     Completed Date:  1991-11-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008358     Medline TA:  J Recept Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  831-48     Citation Subset:  IM    
Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Belgium.
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MeSH Terms
Adenylate Cyclase / chemistry
Amino Acid Sequence
Cell Line
Cross-Linking Reagents
Enzyme Activation
Lymphoma / chemistry*,  enzymology
Molecular Sequence Data
Peptides / chemistry*
Receptors, Gastrointestinal Hormone / chemistry*
Receptors, Vasoactive Intestinal Peptide
Succinimides / chemistry
Tumor Cells, Cultured
Vasoactive Intestinal Peptide / chemistry*
Venoms / chemistry*
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Peptides; 0/Receptors, Gastrointestinal Hormone; 0/Receptors, Vasoactive Intestinal Peptide; 0/Succinimides; 0/Venoms; 37221-79-7/Vasoactive Intestinal Peptide; 57683-72-4/bis(2-(succinimidooxycarbonyloxy)ethyl)sulfone; 89468-62-2/heliodermin; EC Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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