Document Detail


Molecular characterization of the fatty alcohol oxidation pathway for wax-ester mobilization in germinated jojoba seeds.
MedLine Citation:
PMID:  23166353     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Jojoba (Simmondsia chinensis) is the only plant species known to use liquid wax esters (WEs) as a primary seed storage reserve. Upon germination, WE hydrolysis releases very-long-chain fatty alcohols, which must be oxidized to fatty acids by the sequential action of a fatty alcohol oxidase (FAO) and a fatty aldehyde dehydrogenase (FADH) before they can be β-oxidized. Here, we describe the cloning and characterization of genes for each of these two activities. Jojoba FAO and FADH are 52% and 68% identical to Arabidopsis (Arabidopsis thaliana) FAO3 and ALDH3H1, respectively. The genes are expressed most strongly in the cotyledons of jojoba seedlings following germination, but transcripts can also be detected in vegetative tissues. Proteomic analysis indicated that the FAO and FADH proteins can be detected on wax bodies, but they localized to the endoplasmic reticulum when they were expressed as amino-terminal green fluorescent protein fusions in tobacco (Nicotiana tabacum) leaves. Recombinant jojoba FAO and FADH proteins are active on very-long-chain fatty alcohol and fatty aldehyde substrates, respectively, and have biochemical properties consistent with those previously reported in jojoba cotyledons. Coexpression of jojoba FAO and FADH in Arabidopsis enhanced the in vivo rate of fatty alcohol oxidation more than 4-fold. Taken together, our data suggest that jojoba FAO and FADH constitute the very-long-chain fatty alcohol oxidation pathway that is likely to be necessary for efficient WE mobilization following seed germination.
Authors:
Alex S Rajangam; Satinder K Gidda; Christian Craddock; Robert T Mullen; John M Dyer; Peter J Eastmond
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-19
Journal Detail:
Title:  Plant physiology     Volume:  161     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-31     Completed Date:  2013-06-18     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  72-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alcohol Oxidoreductases / genetics,  metabolism
Aldehyde Oxidoreductases / metabolism
Angiosperms / enzymology,  genetics
Arabidopsis / enzymology,  genetics
Cloning, Molecular
Cotyledon / genetics,  metabolism
Endoplasmic Reticulum / metabolism
Enzyme Activation
Esters / metabolism
Fatty Alcohols / metabolism*
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Genes, Plant
Germination*
Oxidation-Reduction
Plant Leaves / enzymology,  genetics
Plants, Genetically Modified / enzymology,  genetics
Proteomics / methods
Recombinant Fusion Proteins / genetics,  metabolism
Seeds / genetics,  metabolism*
Tobacco / enzymology,  genetics
Waxes / metabolism*
Chemical
Reg. No./Substance:
0/Esters; 0/Fatty Alcohols; 0/Recombinant Fusion Proteins; 0/Waxes; 61789-91-1/jojoba wax; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.3.13/alcohol oxidase; EC 1.2.-/Aldehyde Oxidoreductases; EC 1.2.1.48/long-chain-aldehyde dehydrogenase
Comments/Corrections

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