Document Detail


Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis.
MedLine Citation:
PMID:  18407297     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Taurine and its transporter (TauT) are expressed in preimplantation embryos, but their role in embryogenesis is not known. To investigate the role of TauT during embryonic development, we cloned and functionally characterized the zebrafish TauT. The zebrafish TauT cDNA codes for a protein of 625 amino acids which is highly homologous to mammalian TauT. When expressed in mammalian cells, zebrafish TauT mediates taurine uptake in a Na(+)/Cl(-)-dependent manner with a Na(+):Cl(-):taurine stoichiometry of 2:1:1. In the zebrafish embryo, taurine and TauT mRNA are present during early cleavage stages, indicating that both the transporter and its substrate are maternally derived. During embryogenesis, zygotic expression of TauT mRNA is evident in the retina, brain, heart, kidney, and blood vessels. Knockdown of TauT by antisense morpholino oligonucleotides leads to cell death in the central nervous system and increased mortality. These findings suggest a specific role for TauT during development in vertebrates.
Authors:
David J Kozlowski; Zhong Chen; Lina Zhuang; You-Jun Fei; Sammy Navarre; Vadivel Ganapathy
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Publication Detail:
Type:  Journal Article     Date:  2008-03-08
Journal Detail:
Title:  Life sciences     Volume:  82     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-28     Completed Date:  2008-07-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1004-11     Citation Subset:  IM    
Affiliation:
Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912, USA. dkozlowski@mcg.edu
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Apoptosis / drug effects
Cloning, Molecular
Embryonic Development* / drug effects
Epithelial Cells / metabolism
Humans
In Situ Hybridization
Membrane Glycoproteins / biosynthesis*,  genetics
Membrane Transport Proteins / biosynthesis*,  genetics
Molecular Sequence Data
Oligonucleotides, Antisense / pharmacology
Sequence Homology, Amino Acid
Substrate Specificity
Zebrafish / embryology*,  metabolism
Chemical
Reg. No./Substance:
0/Membrane Glycoproteins; 0/Membrane Transport Proteins; 0/Oligonucleotides, Antisense; 148686-53-7/taurine transporter

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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