| Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis. | |
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MedLine Citation:
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PMID: 18407297 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Taurine and its transporter (TauT) are expressed in preimplantation embryos, but their role in embryogenesis is not known. To investigate the role of TauT during embryonic development, we cloned and functionally characterized the zebrafish TauT. The zebrafish TauT cDNA codes for a protein of 625 amino acids which is highly homologous to mammalian TauT. When expressed in mammalian cells, zebrafish TauT mediates taurine uptake in a Na(+)/Cl(-)-dependent manner with a Na(+):Cl(-):taurine stoichiometry of 2:1:1. In the zebrafish embryo, taurine and TauT mRNA are present during early cleavage stages, indicating that both the transporter and its substrate are maternally derived. During embryogenesis, zygotic expression of TauT mRNA is evident in the retina, brain, heart, kidney, and blood vessels. Knockdown of TauT by antisense morpholino oligonucleotides leads to cell death in the central nervous system and increased mortality. These findings suggest a specific role for TauT during development in vertebrates. |
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Authors:
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David J Kozlowski; Zhong Chen; Lina Zhuang; You-Jun Fei; Sammy Navarre; Vadivel Ganapathy |
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Publication Detail:
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Type: Journal Article Date: 2008-03-08 |
Journal Detail:
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Title: Life sciences Volume: 82 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-28 Completed Date: 2008-07-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: England |
Other Details:
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Languages: eng Pagination: 1004-11 Citation Subset: IM |
Affiliation:
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Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912, USA. dkozlowski@mcg.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Apoptosis / drug effects Cloning, Molecular Embryonic Development* / drug effects Epithelial Cells / metabolism Humans In Situ Hybridization Membrane Glycoproteins / biosynthesis*, genetics Membrane Transport Proteins / biosynthesis*, genetics Molecular Sequence Data Oligonucleotides, Antisense / pharmacology Sequence Homology, Amino Acid Substrate Specificity Zebrafish / embryology*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Membrane Glycoproteins; 0/Membrane Transport Proteins; 0/Oligonucleotides, Antisense; 148686-53-7/taurine transporter |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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