Document Detail

Molecular characterization and expression of angiopoietin-like protein 3 in the chicken, Gallus gallus.
MedLine Citation:
PMID:  18602396     Owner:  NLM     Status:  MEDLINE    
Angiopoietin-like protein 3 (ANGPTL3) is a 70 kDa secretory glycoprotein, which has been identified as a plasma triacylglycerol (TG)-increasing factor in KK/San mice. In this study, we have characterized the expression profile and activity of ANGPTL3 in the chicken, Gallus gallus. This is the first report of this protein in a pre-mammalian species and reveals novel details about the molecular structure of, and specific features of the expression of, important molecules in lipid metabolism. Recombinant chicken ANGPTL3 inhibited chicken lipoprotein lipase activity in vitro and increased plasma TG concentrations in vivo. Chicken ANGPTL3 mRNA expression was detected only in the liver. ANGPTL3 mRNA expression was significantly higher in mature (i.e. egg producing) chickens than in immature chickens. However, this increase was not associated with estradiol, although estradiol does enhance triacylglycerol and very low density lipoprotein secretion by the chicken liver. Changes in chicken ANGPTL3 mRNA expression with nutritional state were examined. ANGPLT3 expression was affected by certain nutritional treatments, i.e. fasting and re-feeding, and ANGPTL3 gene expression in the livers of chickens fed a cholesterol-supplemented diet was lower than that in the livers of chickens fed a control diet. The present study is the first to molecularly characterize and analyze the gene expression of ANGPTL3, and suggests that in avian species ANGPTL3 plays a key role in lipid delivery to the yolk.
Kan Sato; Hyang Sook Seol; Kumiko Sato; Tosihiko Kamada; Yukio Akiba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-17
Journal Detail:
Title:  General and comparative endocrinology     Volume:  158     ISSN:  1095-6840     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-29     Completed Date:  2008-10-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  102-7     Citation Subset:  IM    
Animal Science, Department of Biological Production, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
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MeSH Terms
Amino Acid Sequence
Angiopoietins / genetics*,  metabolism
COS Cells
Cercopithecus aethiops
Chickens / genetics*,  metabolism
Cloning, Molecular
Egg Yolk / metabolism
Estradiol / pharmacology
Gene Expression Regulation, Developmental
Lipid Metabolism / genetics
Lipoprotein Lipase / metabolism
Liver / drug effects,  metabolism
Molecular Sequence Data
RNA, Messenger / metabolism
Sequence Homology, Amino Acid
Tissue Distribution
Reg. No./Substance:
0/Angiopoietins; 0/RNA, Messenger; 50-28-2/Estradiol; EC Lipase

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