Document Detail

Molecular characterization of the erythromycin resistance plasmid pPV142 from Staphylococcus simulans.
MedLine Citation:
PMID:  9742700     Owner:  NLM     Status:  MEDLINE    
The 2.5-kb erythromycin resistance (EmR) plasmid pPV142 of Staphylococcus simulans 13044 was isolated and characterized. Sequence analysis identified ORF1 and ORF2 encoding a 158-residue replication protein (Rep142) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransferase), respectively. Structural analysis and Southern hybridization showed that the rep and ermM genes in pPV142 shared homology with the EmR plasmid pPV141 (2.4 kb) of S. chromogenes 3688 and other EmR plasmids known to exist in staphylococci and bacilli. Based on the presence of a 61-bp repeat upstream of the ermM gene, pPV142 is apparently a unique member of the pSN2 family of EmR plasmid able to express erythromycin resistance constitutively.
G A Somkuti; D K Solaiman; D H Steinberg
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  165     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-10-27     Completed Date:  1998-10-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  281-8     Citation Subset:  IM    
U.S. Department of Agriculture, ARS, Eastern Regional Research Center, Wyndmoor, PA 19038, USA.
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MeSH Terms
Amino Acid Sequence
Anti-Bacterial Agents / pharmacology*
Base Sequence
Drug Resistance, Microbial / genetics*
Erythromycin / pharmacology*
Genes, Bacterial
Methyltransferases / chemistry,  genetics*
Molecular Sequence Data
R Factors / genetics*,  isolation & purification
Restriction Mapping
Sequence Alignment
Sequence Analysis, DNA
Staphylococcus / drug effects,  genetics*
Reg. No./Substance:
0/Anti-Bacterial Agents; 114-07-8/Erythromycin; EC 2.1.1.-/Methyltransferases; EC

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