| Molecular characterization of a calmodulin involved in the signal transduction chain of gravitaxis in Euglena gracilis. | |
MedLine Citation:
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PMID: 20213123 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The unicellular flagellate Euglena gracilis shows a negative gravitactic behavior. This is based on physiological mechanisms which in the past have been indirectly assessed. Meanwhile, it was possible to isolate genes involved in the signal transduction chain of gravitaxis. The DNA sequences of five calmodulins were found in Euglena, one of which was only known in its protein structure (CaM.1); the other four are new. The biosynthesis of the corresponding proteins of CaM.1-CaM.5 was inhibited by means of RNA interference to determine their involvement in the gravitactic signal transduction chain. RNAi of CaM.1 inhibits free swimming of the cells and pronounced cell-form aberrations. The division of cells was also hampered. After recovery from RNAi the cell showed precise negative gravitaxis again. Blockage of CaM.3 to CaM. 5 did not impair gravitaxis. In contrast, the blockage of CaM.2 has only a transient and not pronounced influence on motility and cell form, but leads to a total loss of gravitactic orientation for more than 30 days. This indicates that CaM.2 is an element in the signal transduction chain of gravitaxis in E. gracilis. The results are discussed with regard to the current working model of gravitaxis in E. gracilis. |
Authors:
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Viktor Daiker; Michael Lebert; Peter Richter; Donat-Peter H?der |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-06 |
Journal Detail:
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Title: Planta Volume: 231 ISSN: 1432-2048 ISO Abbreviation: Planta Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-18 Completed Date: 2010-06-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1250576 Medline TA: Planta Country: Germany |
Other Details:
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Languages: eng Pagination: 1229-36 Citation Subset: IM |
Affiliation:
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Department of Biology, Plant Ecophysiology, Friedrich-Alexander University, Erlangen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calmodulin
/
genetics,
metabolism* Euglena gracilis / cytology, genetics, physiology* Gene Expression Regulation Gravity Sensing / physiology* Protozoan Proteins / genetics, metabolism* RNA Interference Reverse Transcriptase Polymerase Chain Reaction Signal Transduction* |
| Chemical | |
Reg. No./Substance:
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0/Calmodulin; 0/Protozoan Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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