Document Detail


Molecular characterization of a calmodulin involved in the signal transduction chain of gravitaxis in Euglena gracilis.
MedLine Citation:
PMID:  20213123     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The unicellular flagellate Euglena gracilis shows a negative gravitactic behavior. This is based on physiological mechanisms which in the past have been indirectly assessed. Meanwhile, it was possible to isolate genes involved in the signal transduction chain of gravitaxis. The DNA sequences of five calmodulins were found in Euglena, one of which was only known in its protein structure (CaM.1); the other four are new. The biosynthesis of the corresponding proteins of CaM.1-CaM.5 was inhibited by means of RNA interference to determine their involvement in the gravitactic signal transduction chain. RNAi of CaM.1 inhibits free swimming of the cells and pronounced cell-form aberrations. The division of cells was also hampered. After recovery from RNAi the cell showed precise negative gravitaxis again. Blockage of CaM.3 to CaM. 5 did not impair gravitaxis. In contrast, the blockage of CaM.2 has only a transient and not pronounced influence on motility and cell form, but leads to a total loss of gravitactic orientation for more than 30 days. This indicates that CaM.2 is an element in the signal transduction chain of gravitaxis in E. gracilis. The results are discussed with regard to the current working model of gravitaxis in E. gracilis.
Authors:
Viktor Daiker; Michael Lebert; Peter Richter; Donat-Peter H?der
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-06
Journal Detail:
Title:  Planta     Volume:  231     ISSN:  1432-2048     ISO Abbreviation:  Planta     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-18     Completed Date:  2010-06-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1229-36     Citation Subset:  IM    
Affiliation:
Department of Biology, Plant Ecophysiology, Friedrich-Alexander University, Erlangen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Calmodulin / genetics,  metabolism*
Euglena gracilis / cytology,  genetics,  physiology*
Gene Expression Regulation
Gravity Sensing / physiology*
Protozoan Proteins / genetics,  metabolism*
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction*
Chemical
Reg. No./Substance:
0/Calmodulin; 0/Protozoan Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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