| Molecular characterization of a cDNA encoding DRE-binding transcription factor from dehydration-treated fibrous roots of sweetpotato. | |
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MedLine Citation:
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PMID: 18006323 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new dehydration responsive element-binding (DREB) protein gene encoding for an AP2/EREBP-type transcription factor was isolated by screening of the cDNA library for dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas). Its cDNA (referred to as swDREB1) fragment of 1206bp was sequenced from, which a 257 amino acid residue protein was deduced with a predicted molecular weight of 28.17kDa. A search of the protein BLAST database revealed that this protein can be classified as a typical member of a DREB subfamily. RT-PCR and northern analyses revealed diverse expression patterns of the swDREB1 gene in various tissues of intact sweetpotato plant, and in leaves and fibrous roots exposed to different stresses. The swDREB1 gene was highly expressed in stems and tuberous roots. In fibrous roots, its mRNA accumulation profiles clearly showed strong expression under various abiotic stress conditions such as dehydration, chilling, salt, methyl viologen (MV), and cadmium (Cd) treatment, whereas it did not respond to abscisic acid (ABA) or copper (Cu) treatment. The above results indicate that swDREB1 may be involved in the process of the plant response to diverse abiotic stresses through an ABA-independent pathway. |
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Authors:
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Yun-Hee Kim; Kyoung-Sil Yang; Sun-Hwa Ryu; Kee-Yeun Kim; Wan-Keun Song; Suk-Yoon Kwon; Haeng-Soon Lee; Jae-Wook Bang; Sang-Soo Kwak |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-05 |
Journal Detail:
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Title: Plant physiology and biochemistry : PPB / Société française de physiologie végétale Volume: 46 ISSN: 0981-9428 ISO Abbreviation: Plant Physiol. Biochem. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-18 Completed Date: 2008-07-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882449 Medline TA: Plant Physiol Biochem Country: France |
Other Details:
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Languages: eng Pagination: 196-204 Citation Subset: IM |
Affiliation:
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Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abscisic Acid
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pharmacology Amino Acid Sequence Base Sequence DNA, Complementary / genetics* Gene Expression Regulation, Plant / drug effects Ipomoea batatas / drug effects, genetics*, metabolism Molecular Sequence Data Phylogeny Plant Growth Regulators / pharmacology Plant Leaves / drug effects, genetics, metabolism Plant Proteins / genetics*, metabolism Plant Roots / drug effects, genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Transcription Factors / classification, genetics*, metabolism Water / metabolism, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Plant Growth Regulators; 0/Plant Proteins; 0/Transcription Factors; 21293-29-8/Abscisic Acid; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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