Document Detail

Molecular characterization of a cDNA encoding DRE-binding transcription factor from dehydration-treated fibrous roots of sweetpotato.
MedLine Citation:
PMID:  18006323     Owner:  NLM     Status:  MEDLINE    
A new dehydration responsive element-binding (DREB) protein gene encoding for an AP2/EREBP-type transcription factor was isolated by screening of the cDNA library for dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas). Its cDNA (referred to as swDREB1) fragment of 1206bp was sequenced from, which a 257 amino acid residue protein was deduced with a predicted molecular weight of 28.17kDa. A search of the protein BLAST database revealed that this protein can be classified as a typical member of a DREB subfamily. RT-PCR and northern analyses revealed diverse expression patterns of the swDREB1 gene in various tissues of intact sweetpotato plant, and in leaves and fibrous roots exposed to different stresses. The swDREB1 gene was highly expressed in stems and tuberous roots. In fibrous roots, its mRNA accumulation profiles clearly showed strong expression under various abiotic stress conditions such as dehydration, chilling, salt, methyl viologen (MV), and cadmium (Cd) treatment, whereas it did not respond to abscisic acid (ABA) or copper (Cu) treatment. The above results indicate that swDREB1 may be involved in the process of the plant response to diverse abiotic stresses through an ABA-independent pathway.
Yun-Hee Kim; Kyoung-Sil Yang; Sun-Hwa Ryu; Kee-Yeun Kim; Wan-Keun Song; Suk-Yoon Kwon; Haeng-Soon Lee; Jae-Wook Bang; Sang-Soo Kwak
Related Documents :
8069103 - Ribonuclease activity of petunia inflata s proteins is essential for rejection of self-...
19888823 - The medicago truncatula n5 gene encoding a root-specific lipid transfer protein is requ...
16455083 - Microtubule binding proteins clip-170, eb1, and p150glued form distinct plus-end comple...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-05
Journal Detail:
Title:  Plant physiology and biochemistry : PPB / Société française de physiologie végétale     Volume:  46     ISSN:  0981-9428     ISO Abbreviation:  Plant Physiol. Biochem.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-18     Completed Date:  2008-07-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882449     Medline TA:  Plant Physiol Biochem     Country:  France    
Other Details:
Languages:  eng     Pagination:  196-204     Citation Subset:  IM    
Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Abscisic Acid / pharmacology
Amino Acid Sequence
Base Sequence
DNA, Complementary / genetics*
Gene Expression Regulation, Plant / drug effects
Ipomoea batatas / drug effects,  genetics*,  metabolism
Molecular Sequence Data
Plant Growth Regulators / pharmacology
Plant Leaves / drug effects,  genetics,  metabolism
Plant Proteins / genetics*,  metabolism
Plant Roots / drug effects,  genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Transcription Factors / classification,  genetics*,  metabolism
Water / metabolism,  pharmacology
Reg. No./Substance:
0/DNA, Complementary; 0/Plant Growth Regulators; 0/Plant Proteins; 0/Transcription Factors; 21293-29-8/Abscisic Acid; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Imine derivatives as new potent and selective CB2 cannabinoid receptor agonists with an analgesic ac...
Next Document:  Molecular cloning, characterization and expression analysis of three aldehyde oxidase genes from Pis...