Document Detail


Molecular characterization of a cDNA encoding DRE-binding transcription factor from dehydration-treated fibrous roots of sweetpotato.
MedLine Citation:
PMID:  18006323     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new dehydration responsive element-binding (DREB) protein gene encoding for an AP2/EREBP-type transcription factor was isolated by screening of the cDNA library for dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas). Its cDNA (referred to as swDREB1) fragment of 1206bp was sequenced from, which a 257 amino acid residue protein was deduced with a predicted molecular weight of 28.17kDa. A search of the protein BLAST database revealed that this protein can be classified as a typical member of a DREB subfamily. RT-PCR and northern analyses revealed diverse expression patterns of the swDREB1 gene in various tissues of intact sweetpotato plant, and in leaves and fibrous roots exposed to different stresses. The swDREB1 gene was highly expressed in stems and tuberous roots. In fibrous roots, its mRNA accumulation profiles clearly showed strong expression under various abiotic stress conditions such as dehydration, chilling, salt, methyl viologen (MV), and cadmium (Cd) treatment, whereas it did not respond to abscisic acid (ABA) or copper (Cu) treatment. The above results indicate that swDREB1 may be involved in the process of the plant response to diverse abiotic stresses through an ABA-independent pathway.
Authors:
Yun-Hee Kim; Kyoung-Sil Yang; Sun-Hwa Ryu; Kee-Yeun Kim; Wan-Keun Song; Suk-Yoon Kwon; Haeng-Soon Lee; Jae-Wook Bang; Sang-Soo Kwak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-05
Journal Detail:
Title:  Plant physiology and biochemistry : PPB / Société française de physiologie végétale     Volume:  46     ISSN:  0981-9428     ISO Abbreviation:  Plant Physiol. Biochem.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-18     Completed Date:  2008-07-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882449     Medline TA:  Plant Physiol Biochem     Country:  France    
Other Details:
Languages:  eng     Pagination:  196-204     Citation Subset:  IM    
Affiliation:
Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / pharmacology
Amino Acid Sequence
Base Sequence
DNA, Complementary / genetics*
Gene Expression Regulation, Plant / drug effects
Ipomoea batatas / drug effects,  genetics*,  metabolism
Molecular Sequence Data
Phylogeny
Plant Growth Regulators / pharmacology
Plant Leaves / drug effects,  genetics,  metabolism
Plant Proteins / genetics*,  metabolism
Plant Roots / drug effects,  genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Transcription Factors / classification,  genetics*,  metabolism
Water / metabolism,  pharmacology
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Plant Growth Regulators; 0/Plant Proteins; 0/Transcription Factors; 21293-29-8/Abscisic Acid; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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