Document Detail


Molecular characterization of atypical Chlamydia and evidence of their dissemination in different European and Asian chicken flocks by specific real-time PCR.
MedLine Citation:
PMID:  22690809     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chlamydia psittaci is a zoonotic pathogen associated primarily with avian chlamydiosis. New chlamydial agents with suspected zoonotic potential were recently detected from domestic poultry in Germany and France indicating that the spectrum of Chlamydiaceae encountered in birds is not confined to a single chlamydial species. For further characterization, a specific real-time PCR targeting the conserved 16S rRNA gene was developed and validated for a specific detection of these atypical Chlamydiaceae. In order to address the epidemiological importance of the new chlamydial agents and their distribution, Chlamydiaceae-positive chicken samples collected from flocks from five different countries were examined. The results confirmed that C. psittaci is not the predominant chlamydial species among chickens examined and suggested that the new chlamydial agents could putatively be widespread in poultry flocks (France, Greece, Croatia, Slovenia and China at least) justifying their systematic investigation when poultry samples are submitted to laboratories for avian chlamydiosis diagnosis. Besides, 16S rRNA-based dendrogram, including sequences from both isolates of the new chlamydial agents or positive samples as well as representative sequences from species belonging to the order Chlamydiales, showed the new chlamydial agents to form a distinct line of descent separated from those of other chlamydial species, but clearly grouped within the family Chlamydiaceae. Finally, the phylogenetic tree inferred from the multi-locus sequence typing based on four housekeeping fragments (gatA, gidA, enoA and hflX) and the ompA-based dendrogram showed an almost identical topology of the new chlamydial agents with that recovered by 16S rRNA-based dendrogram. Interestingly, partial ompA gene sequences displayed considerable diversity among isolates.
Authors:
Aleksandar Zocevic; Fabien Vorimore; Cvetka Marhold; Danijela Horvatek; Dongying Wang; Brigita Slavec; Zoi Prentza; Grigorios Stavianis; Estella Prukner-Radovcic; Alenka Dovc; Victoria I Siarkou; Karine Laroucau
Related Documents :
24517729 - Use of an improved atpa amplification and sequencing method to identify members of the ...
8550579 - Molecular cloning, heterologous expression, and characterization of human glyoxalase ii.
23559859 - Detection of novel mutations that cause autosomal dominant retinitis pigmentosa in cand...
12058179 - Cloning of metallotionein cdnas and its gene in shore crab(carcinus maenas).
22514089 - Activation and epigenetic regulation of dna transposon ndart1 in rice.
19160069 - Molecular cloning and characterization of a novel gene encoding zinc finger protein fro...
3356709 - Human profilin. molecular cloning, sequence comparison, and chromosomal analysis.
8075639 - Striking sequence similarity over almost 100 kilobases of human and mouse t-cell recept...
10543399 - Characterisation of the human snail (snai1) gene and exclusion as a major disease gene ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-12
Journal Detail:
Title:  Environmental microbiology     Volume:  -     ISSN:  1462-2920     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.
Affiliation:
Bacterial Zoonoses Unit, French Agency for Food, Environmental & Occupational Health Safety (Anses), Maisons-Alfort, France Institute for Health Care of Poultry, Veterinary faculty, University in Ljubljana, Slovenia Faculty of Veterinary Medicine, University of Zagreb, Department of Poultry Diseases with Clinic, Zagreb, Croatia Parasitology Department, College of Animal Science and Technology, Guangxi University, Nanning, China Laboratory of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inclusion of Chloromethane Guests Affects Conformation and Internal Dynamics of Cryptophane-D Host.
Next Document:  Chemical inactivation of protein toxins on food contact surfaces.