Document Detail

Molecular characterization of Syrian date palm cultivars using plasmid-like DNA markers.
MedLine Citation:
PMID:  22568006     Owner:  NLM     Status:  In-Process    
Date palm (Phoenix dactylifera L.) is one of the most important domesticated fruit trees in the Near East and North African countries. This tree has been, for several decades, in serious threat of being completely destroyed by the "Bayoud" disease caused by Fusarium oxysporum f. sp. albedinis. In this study, 18 Syrian date palm cultivars and four male trees were analyzed according to the identity of mitochondrial plasmid-like DNAs. A PCR strategy that employs plasmid-like DNAs-specific primer pair was used. These primers amplify a product of either 373-bp or 265-bp that corresponds to the S-(Bayoud-susceptible) or the R-plasmid (Bayoud-resistant), respectively. Generated data revealed that only six cultivars ('Medjool', 'Ashrasi', 'Gish Rabi', 'Khineze', and yellow- and red-'Kabkab') have the S-plasmid, suggesting their susceptibility to the fusariosis, while the remaining 12 cultivars and the four male trees contain the R-plasmid, suggesting their resistance to the fusariosis. The PCR process applied here has been proved efficient for the rapid screening for the presence of the S and R DNAs in Syrian date palm. PCR markers developed in this study could be useful for the screening of date palm lines growing in the field. The availability of such diagnostic tool for plasmid characterization in date palm would also be of great importance in establishing propagation and breeding programs of date palm in Syria.
N Haider; I Nabulsi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genetika     Volume:  48     ISSN:  0016-6758     ISO Abbreviation:  Genetika     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-05-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0047354     Medline TA:  Genetika     Country:  Russia (Federation)    
Other Details:
Languages:  eng     Pagination:  270-4     Citation Subset:  IM    
Department of Molecular Biology and Biotechnology, AECS, P.O. Box 6091, Damascus, Syria
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