Document Detail

Molecular characterization of the SEC1 gene of Saccharomyces cerevisiae: subcellular distribution of a protein required for yeast protein secretion.
MedLine Citation:
PMID:  8368004     Owner:  NLM     Status:  MEDLINE    
Strains of Saccharomyces cerevisiae harbouring temperature-sensitive mutations in the SEC1 and SEC5 genes exhibit an accumulation of post-Golgi secretory vesicles at 37 degrees C. We have cloned a fragment of yeast DNA which carries two distinct genes, one of which complements a sec1 mutation, and the other a sec5 mutation. Genetic test confirm that the sec1-complementing gene is indeed SEC1, and is essential for cell growth. Nucleotide sequence analysis reveals that the cloned SEC1 gene is the same as a previously sequenced sec1-complementing gene. The SEC1 sequence encodes a protein of 724 amino acids with a predicted molecular mass of 83 kDa. Antibodies purified from a polyclonal antiserum raised against the protein product of the cloned gene recognize a yeast protein of apparent molecular mass 78 kDa which is found in a detergent-resistant association with a rapidly sedimenting yeast subcellular fraction, behaviour which is suggestive of an interaction with a component of the yeast cytoskeleton.
M Egerton; J Zueco; A Boyd
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  9     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-10-06     Completed Date:  1993-10-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  703-13     Citation Subset:  IM    
Department of Biochemistry, University of Edinburgh Medical School, U.K.
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MeSH Terms
Cloning, Molecular
Fungal Proteins / genetics*,  secretion*
Genes, Fungal / genetics*,  physiology
Munc18 Proteins
Mutation / genetics,  physiology
Nerve Tissue Proteins*
Restriction Mapping
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae Proteins
Vesicular Transport Proteins*
Reg. No./Substance:
0/Fungal Proteins; 0/Munc18 Proteins; 0/Nerve Tissue Proteins; 0/SEC1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Vesicular Transport Proteins

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